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PXD006120

PXD006120 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExtracellular matrix downregulation in the Drosophila heart preserves contractile function and improves lifespan
DescriptionAging is associated with extensive remodeling of the heart, including basement membrane (BM) components that surround cardiomyocytes. Remodeling is thought to impair cardiac mechanotransduction, but the contribution of specific BM components to age-related lateral communication between cardiomyocytes is unclear. Using a genetically tractable, rapidly aging model with sufficient cardiac genetic homology and morphology, e.g. Drosophila melanogaster, we observed differential regulation of BM collagens between laboratory strains, correlating with changes in muscle physiology leading to cardiac dysfunction. Therefore, we sought to understand the extent to which BM proteins modulate contractile function during aging. Cardiac-restricted knockdown of ECM genes Pericardin, Laminin A, and Viking in Drosophila prevented age-associated heart tube restriction and increased contractility, even under viscous load. Most notably, reduction of Laminin A expression correlated with an overall preservation of contractile velocity with age and extension of organismal lifespan. Global heterozygous knockdown confirmed these data, which provides new evidence of a direct link between BM homeostasis, contractility, and maintenance of lifespan.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:04:42.765.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKoen Raedschelders
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-03-20 04:36:36ID requested
12017-03-27 01:40:24announced
22024-10-22 04:04:43announced2024-10-22: Updated project metadata.
Publication List
Sessions AO, Kaushik G, Parker S, Raedschelders K, Bodmer R, Van Eyk JE, Engler AJ, Extracellular matrix downregulation in the Drosophila heart preserves contractile function and improves lifespan. Matrix Biol, 62():15-27(2017) [pubmed]
10.1016/j.matbio.2016.10.008;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Basement membrane, Laminin A,Aging, Extracellular matrix, Drosophila
Contact List
Jennifer Van Eyk
contact affiliationCedars-Sinai Medical Center, Heart Institute, Los Angeles, CA. United States of America
contact emailjennifer.vaneyk@cshs.org
lab head
Koen Raedschelders
contact affiliationCedars-Sinai Medical Center
contact emailkoen.raedschelders@cshs.org
dataset submitter
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Dataset FTP location
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PRIDE project URI
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