PXD006117

PXD006117 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomic Analysis of the Mycobacterium tuberculosis Lineage 7 Reveals Differentially Abundant Proteins Linked to Slow Growth
Description	Differentially abundant proteins were quantified in the slowly growing Mycobacterium tuberculosis (M. tuberculosis) lineage 7 strains and the M. tuberculosis lineage 4 reference strain H37Rv. Mass spectrometry based proteomic analysis was employed to identify, quantitate and compare the protein profiles of strains from the two M. tuberculosis lineages (lineage 7 and 4). Label-free peptide quantification of whole cells from M. tuberculosis lineage 7 and 4 yielded the identification of 2825 and 2541 proteins, respectively. A combined total of 2867 protein groups covering 71% of the predicted M. tuberculosis proteome were identified. Comparative proteomic mapping of M. tuberculosis lineage 7 and 4 strains showed that the abundance of 125 proteins was significantly altered. Functional analysis showed that a number of M. tuberculosis proteins involved in growth and virulence were less abundant in lineage 7 compared to lineage 4. Five ABC transporter proteins, three phosphate binding proteins essential for inorganic phosphate uptake, and six components of the type 7 secretion system ESX-3 involved in iron acquisition were less abundant in M. tuberculosis lineage 7. This proteogenomic analysis provided an insight into the lineage 7-specific protein profile which may provide cues to understanding the differential properties of lineage 7 strains in terms of slow growth, survival fitness and pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2017-05-24
AnnouncementXML	Submission_2017-06-07_00:27:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shewit Kalayou
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-20 03:03:08	ID requested
1	2017-05-24 04:47:19	announced
⏵ 2	2017-06-07 00:27:04	announced	Updated publication reference for PubMed record(s): 28536560.

Yimer SA, Birhanu AG, Kalayou S, Riaz T, Zegeye ED, Beyene GT, Holm-Hansen C, Norheim G, Abebe M, Aseffa A, T, ø, njum T, Lineage 7 and Lineage 4 Strains Reveals Differentially Abundant Proteins Linked to Slow Growth and Virulence. Front Microbiol, 8():795(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Mycobacterium tuberculosis, tuberculosis, lineage 7, proteomics, Ethiopia, mass spectrometry, type 7 secretion

Prof Tone Tønjum
contact affiliation	Department of Microbiology,Institute of clinical medicine, University of Oslo, Norway
contact email	tone.tonjum@medisin.uio.no
lab head
Shewit Kalayou
contact affiliation	Genome dynamics group, Oslo University Hospital
contact email	shetek@rr-research.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006117
     1. Label: PRIDE project
     2. Name: Comparative Proteomic Analysis of the Mycobacterium tuberculosis Lineage 7 Reveals Differentially Abundant Proteins Linked to Slow Growth