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PXD006115

PXD006115 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics identification of regulated proteins during cardyomyocyte differentiation
DescriptionThe rat cardio-myoblast cell line H9C2 has emerged as an important tool for studying cardiac development and toxicology. We present here a rigorous proteomic analysis that for the first time studied changes of proteins during H9C2 differentiation into cardiomyocyte-like cells over time. Quantitative mass spectrometry followed by gene ontology (GO) enrichment analysis revealed early changes in protein pathways that enable cardiac muscle morphogenesis/contraction and suggested an involvement of proteins relevant to sphingolipid synthesis. This early differentiation was followed by differentiation-induced alterations in cation transport and beta-oxidation at later time points. Applying a two-way ANOVA to study the temporal profile of H9C2 differentiation in further detail revealed eight clusters of co-regulated proteins that could be associated to early, late, continuous and transient up- and downregulation. Subsequent Reactome pathway analysis based on these eight clusters further corroborated and detailed the results of the GO analysis. Specifically, this analysis confirmed proteins related to pathways in muscle contraction as early and transiently upregulated, and proteins relevant to ECM matrix organization as early downregulated, while changes related to cardiac metabolism occurred at later time points. Our results are in line with a ‘function follows form’ model of differentiation, whereby early and transient changes of cellular morphology enable subsequent changes that are relevant for the characteristic physiology of cardiac cells.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:04:36.113.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterImpens Francis
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-03-20 02:42:41ID requested
12018-11-07 01:00:49announced
22024-10-22 04:04:38announced2024-10-22: Updated project metadata.
Publication List
10.1039/c8mo00036k;
Kankeu C, Clarke K, Van Haver D, Gevaert K, Impens F, Dittrich A, Roderick HL, Passante E, Huber HJ, Quantitative proteomics and systems analysis of cultured H9C2 cardiomyoblasts during differentiation over time supports a 'function follows form' model of differentiation. Mol Omics, 14(3):181-196(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Rattus norvegicus, labelfree shotgun proteomics,cardiac development, H9C2 cells
Contact List
Heinrich Huber
contact affiliationLaboratory for Systems Theory and Automatic Control, Institute for Automation Engineering (IFAT), Otto-von-Guericke University Magdeburg, 39106 Magdeburg, Germany
contact emailheinrich.huber@ovgu.de
lab head
Impens Francis
contact affiliationVIB Proteomics Core Ghent University
contact emailfrancis.impens@vib-ugent.be
dataset submitter
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