PXD006103

PXD006103 is an original dataset announced via ProteomeXchange.

Title	Effect of Sec61 inhibitor mycolactone on the proteome of the MutuDC dendritic cell line
Description	Here we used a newly identified Sec61 blocker, mycolactone, to analyze Sec61 contribution to antigen cross presentation, ERAD and transport of internalized antigens into the cytosol in dendritic cells. Shotgun proteomics were used to asses the broad-ranging effects of Sec61 blockade on dendritic cells and its effect on critical proteins required for antigen presentation and ERAD.
HostingRepository	PRIDE
AnnounceDate	2017-07-11
AnnouncementXML	Submission_2018-06-26_08:31:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jean-David Morel
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	L-methionine sulfoxide; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-16 04:25:41	ID requested
1	2017-07-11 04:39:09	announced
⏵ 2	2018-06-26 08:31:12	announced	Updated publication reference for PubMed record(s): 29915147, 28679634.

Grotzke JE, Kozik P, Morel JD, Impens F, Pietrosemoli N, Cresswell P, Amigorena S, Demangel C, Sec61 blockade by mycolactone inhibits antigen cross-presentation independently of endosome-to-cytosol export. Proc Natl Acad Sci U S A, 114(29):E5910-E5919(2017) [pubmed]

Morel JD, Paatero AO, Wei J, Yewdell JW, Guenin-Mac, é L, Van Haver D, Impens F, Pietrosemoli N, Paavilainen VO, Demangel C, Proteomics Reveals Scope of Mycolactone-mediated Sec61 Blockade and Distinctive Stress Signature. Mol Cell Proteomics, 17(9):1750-1765(2018) [pubmed]

submitter keyword: Mouse MutuDC dendritic mycolactone Sec61 MS/MS LFQ

Caroline DEMANGEL
contact affiliation	Institut Pasteur, Immunobiology of Infection Unit, 75015 Paris, France
contact email	caroline.demangel@pasteur.fr
lab head
Jean-David Morel
contact affiliation	Institut Pasteur, Paris, France
contact email	jean-david.morel@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD006103

PRIDE project URI

• PRIDE
  1. PXD006103
     1. Label: PRIDE project
     2. Name: Effect of Sec61 inhibitor mycolactone on the proteome of the MutuDC dendritic cell line