PXD006102

PXD006102 is an original dataset announced via ProteomeXchange.

Title	Dendrobium officinale seed symbiotic germination iTRAQ
Description	Mycorrhizal fungi colonize orchid seed and induce the germination. This so-called symbiotic germination is a critical developmental process in the lifecycle of all orchids. However, the molecular changes taking place during the orchid seed symbiotic germination still remains largely unknown. To better understand the molecular mechanism of orchid seed germination, we performed comparative transcriptomic and proteomic analysis on Chinese traditional medicinal orchid plants, Dendrobium officinale to explore protein expression change at the different developmental stages between asymbiotic and symbiotic germination and identify the key proteins regulated symbiotic germination of orchid seeds. iTRAQ analysis from 8 samples identified 2256 plant proteins, of which, 308 proteins were differentially expressed across three developmental stages within asymbiotic or symbiotic accession and 229 proteins are differentially expressed in the symbiotic germination compared to asymbiotic germination. 32 proteins are co-upregulated in both proteomic and transcriptomic level for symbiotic germination compared to asymbiotic germination. Our results revealed that symbiotic germination of D. officinale seeds probably shares the common signal pathway with asymbiotic germination during the early germination stage.
HostingRepository	PRIDE
AnnounceDate	2017-05-12
AnnouncementXML	Submission_2017-05-12_01:21:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Chen
SpeciesList	scientific name: Dendrobium sp. XMW-2002-13; NCBI TaxID: 217459;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-16 02:09:05	ID requested
⏵ 1	2017-05-12 01:21:16	announced

Chen J, Liu SS, Kohler A, Yan B, Luo HM, Chen XM, Guo SX, iTRAQ and RNA-Seq Analyses Provide New Insights into Regulation Mechanism of Symbiotic Germination of Dendrobium officinale Seeds (Orchidaceae). J Proteome Res, 16(6):2174-2187(2017) [pubmed]

curator keyword: Biological

submitter keyword: Orchidaceae seed, mycorrhizal symbiosis, iTRAQ

Biotechnological Center
contact affiliation	Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College
contact email	kibchenjuan@126.com
lab head
Juan Chen
contact affiliation	Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences
contact email	kibchenjuan@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006102

PRIDE project URI

• PRIDE
  1. PXD006102
     1. Label: PRIDE project
     2. Name: Dendrobium officinale seed symbiotic germination iTRAQ