PXD006096

PXD006096 is an original dataset announced via ProteomeXchange.

Title	Comparison of Targeted MS Methods in Activity-Based Protein Profiling 2: DIA
Description	To examine the different mass spectrometry approaches to monitoring kinases after enrichment with desthiobiotinylating probes for activity-based protein profiling (ABPP), two experiments were performed with H1993 lung cancer cells. First, cell lysates were pre-treated with DMSO vehicle, dasatinib, or erlotinib prior to addition of the ATP probe for ABPP to compare the differences in kinase labeling associated with examples of kinase inhibitors that vary in target selectivity. Then, to examine changes in cellular signaling, H1993 cells were treated with vehicle controls, BEX-235 (PI3K inhibitor), or Crizotinib. LC-MS/MS using data dependent acquisition, data-independent acquisition (pSMART), parallel reaction monitoring, and selected reaction monitoring (or multiple reaction monitoring) mass spectrometry were used to detect and relatively quantify the desthiobiotinylated kinase peptides.
HostingRepository	PRIDE
AnnounceDate	2017-11-28
AnnouncementXML	Submission_2017-11-28_04:27:42.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006096
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	John Koomen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-15 01:53:08	ID requested
⏵ 1	2017-11-28 04:27:43	announced

Hoffman MA, Fang B, Haura EB, Rix U, Koomen JM, Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res, 17(1):63-75(2018) [pubmed]

curator keyword: Technical

submitter keyword: DDA, DIA, PRM, SRM/MRM, Activity-Based Protein Profiling, Lung Cancer

John Koomen, PhD
contact affiliation	Moffitt Cancer Center
contact email	john.koomen@moffitt.org
lab head
John Koomen
contact affiliation	Moffitt Cancer Center
contact email	john.koomen@moffitt.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD006096

PRIDE project URI

• PRIDE
  1. PXD006096
     1. Label: PRIDE project
     2. Name: Comparison of Targeted MS Methods in Activity-Based Protein Profiling 2: DIA