PXD006080

PXD006080 is an original dataset announced via ProteomeXchange.

Title	Ncd regulation reveals a new mechanism for targeting proteins to the meiotic spindle in oocytes
Description	The meiotic spindle in oocytes is formed without centrosomes. How a bipolar spindle is assembled and maintained around chromosomes in oocytes remains to be established. Multiple kinases are known to regulate the meiotic spindle, but how they execute their function is poorly understood. We found that the phospho-docking protein 14-3-3ε, together with the other isoform ζ, stabilises spindle bipolarity in Drosophila oocytes. A critical 14-3-3 target is the minus-end directed motor Ncd (human HSET; kinesin-14) which has well documented roles in stabilising a bipolar spindle in oocytes. Phospho-docking by 14-3-3 inhibits the microtubule binding activity of the non-motor Ncd tail. Further phosphorylation by Aurora B kinase can release Ncd from this inhibitory effect of 14-3-3. As Aurora B localises to chromosomes and spindles, 14-3-3 facilitates specific association of Ncd with spindle microtubules by preventing Ncd from binding to non-spindle microtubules in oocytes. Therefore, 14-3-3 translates a spatial cue provided by Aurora B to target Ncd selectively to the spindle within the large volume of oocytes.
HostingRepository	PRIDE
AnnounceDate	2017-08-29
AnnouncementXML	Submission_2017-09-11_00:49:50.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006080
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Robin Beaven
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-13 03:10:10	ID requested
1	2017-08-29 06:18:04	announced
⏵ 2	2017-09-11 00:49:51	announced	Updated publication reference for PubMed record(s): 28860275.

Beaven R, Bastos RN, Spanos C, Rom, é P, Cullen CF, Rappsilber J, Giet R, Goshima G, Ohkura H, 14-3-3 regulation of Ncd reveals a new mechanism for targeting proteins to the spindle in oocytes. J Cell Biol, 216(10):3029-3039(2017) [pubmed]

curator keyword: Biological

submitter keyword: Ncd regulation, meiosis, meiotic spindle, Drosophila, oocytes, shotgun proteomics

Hiro Ohkura
contact affiliation	Wellcome Centre for Cell biology, University of Edinburgh, UK
contact email	Hiro.Ohkura@ed.ac.uk
lab head
Robin Beaven
contact affiliation	University of Edinburgh
contact email	Robin.Beaven@ed.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006080
     1. Label: PRIDE project
     2. Name: Ncd regulation reveals a new mechanism for targeting proteins to the meiotic spindle in oocytes