PXD006078

PXD006078 is an original dataset announced via ProteomeXchange.

Title	Ovarian Cancer Lipid raft proteomics and role of OPCML
Description	ipid rafts are dynamic membrane micro-domains that orchestrate molecular interactions and are implicated in cancer development. To understand potential role of tumor suppressor OPCML on the altered dynamics of lipid raft residing proteins, we performed quantitative (SILAC) proteomics experiment. We recently found that OPCML negatively regulates a subset of receptor tyrosine kinases (RTKs) by altering their recycling and ubiquitin-mediated degradation via sequestration to lipid rafts; however, the molecular mechanism linking OPCML tumor suppressor expression to altered RTK trafficking remains unclear. Here we performed a quantitative lipid raft proteomics study to dissect raft-mediated mechanism of OPCML-regulated tumor suppression. We also examined raft-associated mechanism of OPCML P95R, a common mutation resulting in substitution of proline to arginine at position 95, mediated regulation of adhesion potential of tumors.
HostingRepository	PRIDE
AnnounceDate	2017-03-16
AnnouncementXML	Submission_2017-03-16_02:43:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anup Shah
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	6520 Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-13 03:03:17	ID requested
⏵ 1	2017-03-16 02:43:20	announced

Shah AD, Inder KL, Shah AK, Cristino AS, McKie AB, Gabra H, Davis MJ, Hill MM, Integrative Analysis of Subcellular Quantitative Proteomics Studies Reveals Functional Cytoskeleton Membrane-Lipid Raft Interactions in Cancer. J Proteome Res, 15(10):3451-3462(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: OPCML, Lipid raft, Skov-3, SILAC, P95R OPCML

Michelle M. Hill
contact affiliation	Associate Professor, Head Cancer Proteomics Group, University of Queensland Diamantina Institute, Woolloongabba, QLD, 4102
contact email	m.hill2@uq.edu.au
lab head
Anup Shah
contact affiliation	University of Queensland
contact email	a.shah3@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD006078

PRIDE project URI

• PRIDE
  1. PXD006078
     1. Label: PRIDE project
     2. Name: Ovarian Cancer Lipid raft proteomics and role of OPCML