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PXD006069

PXD006069 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFunctional marker in glioblastoma
DescriptionTo identify receptors and pathways active in glioblastoma (GBM) stem like cells (GSCs), we generated and screened thousands of monoclonal antibodies (mAbs) for preferential binding to primary cultures enriched in GSCs. This led to the identification of the integrin alpha 7 (ITGA7) as a major laminin receptor in GSCs and in primary high-grade glioma specimens. Analyses of mRNA profiles in comprehensive datasets revealed that high ITGA7 expression was negatively correlated with survival of patients with both low- and high-grade glioma. In vitro and in vivo analyses demonstrated a key biological function of ITGA7 in growth and invasion of GSCs. In addition, we showed that targeting ITGA7 by RNAi or blocking mAbs impaired laminin-induced signaling and led to a significant delay of tumor engraftment and strong reduction in size and invasion. Our data underline the potential value of ITGA7 as glioma biomarker and therapeutic target.
HostingRepositoryPRIDE
AnnounceDate2018-01-10
AnnouncementXMLSubmission_2018-01-10_04:13:03.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterUwe Warnken
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-03-10 05:56:16ID requested
12018-01-10 04:13:04announced
Publication List
Haas TL, Sciuto MR, Brunetto L, Valvo C, Signore M, Fiori ME, di Martino S, Giannetti S, Morgante L, Boe A, Patrizii M, Warnken U, Schn, รถ, lzer M, Ciolfi A, Di Stefano C, Biffoni M, Ricci-Vitiani L, Pallini R, De Maria R, 7 Is a Functional Marker and Potential Therapeutic Target in Glioblastoma. Cell Stem Cell, 21(1):35-50.e9(2017) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: glioblastoma
cancer stem cells
integrin alpha 7
glioma cell invasion
stem cell marker
high throughput antibody screening
Contact List
Martina Schnoelzer
contact affiliationProtein Analysis Core Facility German Cancer Research Center (DKFZ)
contact emailM.Schnoelzer@dkfz.de
lab head
Uwe Warnken
contact affiliationGerman Cancer Research Center
contact emailu.warnken@dkfz.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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