PXD006069 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Functional marker in glioblastoma |
Description | To identify receptors and pathways active in glioblastoma (GBM) stem like cells (GSCs), we generated and screened thousands of monoclonal antibodies (mAbs) for preferential binding to primary cultures enriched in GSCs. This led to the identification of the integrin alpha 7 (ITGA7) as a major laminin receptor in GSCs and in primary high-grade glioma specimens. Analyses of mRNA profiles in comprehensive datasets revealed that high ITGA7 expression was negatively correlated with survival of patients with both low- and high-grade glioma. In vitro and in vivo analyses demonstrated a key biological function of ITGA7 in growth and invasion of GSCs. In addition, we showed that targeting ITGA7 by RNAi or blocking mAbs impaired laminin-induced signaling and led to a significant delay of tumor engraftment and strong reduction in size and invasion. Our data underline the potential value of ITGA7 as glioma biomarker and therapeutic target. |
HostingRepository | PRIDE |
AnnounceDate | 2018-01-10 |
AnnouncementXML | Submission_2018-01-10_04:13:03.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Uwe Warnken |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-03-10 05:56:16 | ID requested | |
⏵ 1 | 2018-01-10 04:13:04 | announced | |
Publication List
Haas TL, Sciuto MR, Brunetto L, Valvo C, Signore M, Fiori ME, di Martino S, Giannetti S, Morgante L, Boe A, Patrizii M, Warnken U, Schn, รถ, lzer M, Ciolfi A, Di Stefano C, Biffoni M, Ricci-Vitiani L, Pallini R, De Maria R, 7 Is a Functional Marker and Potential Therapeutic Target in Glioblastoma. Cell Stem Cell, 21(1):35-50.e9(2017) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: glioblastoma |
cancer stem cells |
integrin alpha 7 |
glioma cell invasion |
stem cell marker |
high throughput antibody screening |
Contact List
Martina Schnoelzer |
contact affiliation | Protein Analysis Core Facility German Cancer Research Center (DKFZ) |
contact email | M.Schnoelzer@dkfz.de |
lab head | |
Uwe Warnken |
contact affiliation | German Cancer Research Center |
contact email | u.warnken@dkfz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006069 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006069
- Label: PRIDE project
- Name: Functional marker in glioblastoma