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PXD006066

PXD006066 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative cardiac phosphoproteomics profiling during ischemic/reperfusion in an immature swine model
DescriptionIschemia/Reperfusion (I/R) results in altered metabolic and molecular responses. Phosphorylation, a posttranslational modification, is one of the most noted regulatory mechanisms and differential phosphorylation affects numerous regulatory and signaling mechanisms. To further understand the phosphoproteomic changes that occur in the heart during I/R we utilized iTRAQ-based quantitative proteomic and LC-MS/MS techniques to obtain a profile of phosphopeptides expressed under control and I/R conditions. A total of 1896 phosphopeptides were identified, and 116 were assessed as significant. The 116 phosphopeptides represented 87 unique proteins. Analysis of the phosphopeptides using Motif-x identified 2 predominant motifs: arginine and proline-directed serine phosphorylation sites. Two proteins known to be altered by I/R were among the dataset obtained, phospholamban and pyruvate dehydrogenase, and were used to confirm validity of the profile. Functional characterization of the phosphopetides identified in this study could lead to further understanding of the signaling mechanisms involved during I/R damage in the heart, as well as identifying new areas to target therapeutic strategies.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:08:42.053.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD006066
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMatthew Monroe
SpeciesList scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationListPhospho; iTRAQ8plex; Carbamidomethyl
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-03-09 03:10:24ID requested
12017-05-02 05:10:20announced
22024-10-22 04:08:50announced2024-10-22: Updated project metadata.
Publication List
Ledee D, Kang MA, Kajimoto M, Purvine S, Brewer H, Pasa-Tolic L, Portman MA, Quantitative cardiac phosphoproteomics profiling during ischemia-reperfusion in an immature swine model. Am J Physiol Heart Circ Physiol, 313(1):H125-H137(2017) [pubmed]
10.1152/ajpheart.00842.2016;
10.6019/PXD006066;
Keyword List
curator keyword: Biomedical
submitter keyword: proteomics, phosphopeptide quantitation, iTRAQ
Contact List
Michael Portman, MD
contact affiliationSeattle Children's Hospital, USA
contact emailmichael.portman@seattlechildrens.org
lab head
Matthew Monroe
contact affiliationPacific Northwest National Laboratory
contact emailmatthew.monroe@pnnl.gov
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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