PXD006066

PXD006066 is an original dataset announced via ProteomeXchange.

Title	Quantitative cardiac phosphoproteomics profiling during ischemic/reperfusion in an immature swine model
Description	Ischemia/Reperfusion (I/R) results in altered metabolic and molecular responses. Phosphorylation, a posttranslational modification, is one of the most noted regulatory mechanisms and differential phosphorylation affects numerous regulatory and signaling mechanisms. To further understand the phosphoproteomic changes that occur in the heart during I/R we utilized iTRAQ-based quantitative proteomic and LC-MS/MS techniques to obtain a profile of phosphopeptides expressed under control and I/R conditions. A total of 1896 phosphopeptides were identified, and 116 were assessed as significant. The 116 phosphopeptides represented 87 unique proteins. Analysis of the phosphopeptides using Motif-x identified 2 predominant motifs: arginine and proline-directed serine phosphorylation sites. Two proteins known to be altered by I/R were among the dataset obtained, phospholamban and pyruvate dehydrogenase, and were used to confirm validity of the profile. Functional characterization of the phosphopetides identified in this study could lead to further understanding of the signaling mechanisms involved during I/R damage in the heart, as well as identifying new areas to target therapeutic strategies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:08:42.053.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006066
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Monroe
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	Phospho; iTRAQ8plex; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-09 03:10:24	ID requested
1	2017-05-02 05:10:20	announced
⏵ 2	2024-10-22 04:08:50	announced	2024-10-22: Updated project metadata.

Ledee D, Kang MA, Kajimoto M, Purvine S, Brewer H, Pasa-Tolic L, Portman MA, Quantitative cardiac phosphoproteomics profiling during ischemia-reperfusion in an immature swine model. Am J Physiol Heart Circ Physiol, 313(1):H125-H137(2017) [pubmed]

10.1152/ajpheart.00842.2016;

10.6019/PXD006066;

curator keyword: Biomedical

submitter keyword: proteomics, phosphopeptide quantitation, iTRAQ

Michael Portman, MD
contact affiliation	Seattle Children's Hospital, USA
contact email	michael.portman@seattlechildrens.org
lab head
Matthew Monroe
contact affiliation	Pacific Northwest National Laboratory
contact email	matthew.monroe@pnnl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006066
     1. Label: PRIDE project
     2. Name: Quantitative cardiac phosphoproteomics profiling during ischemic/reperfusion in an immature swine model