PXD006064

PXD006064 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Glycine max seeds during controlled deterioration treatment
Description	Controlled deterioration treatment (CDT) negatively affects the seed quality and vigor during post-harvest storage. A label-free proteomic approach was utilized to understand the CDT responses in soybean seeds. Soybean seed are rich in seed-storage proteins (SSPs) constituting up to 70 to 80% of the total seed protein content. Due to the presence of these SSPs, it is very difficult to identify and/or characterize the low-abundance regulatory proteins. Availability of appropriate methods for extraction of low-abundance proteins (LAPs) are now providing a platform for the identification of novel proteins involved in the signal perception and transduction during environmental perturbations. To enrich LAPs, the extracted total seed proteins were subjected to protamine sulfate precipitation (PSP) method to deplete SSPs. Fractionated protein fractions thus prepared were analyzed for identification of differential proteins using Label-free quantitative proteomics approaches.
HostingRepository	PRIDE
AnnounceDate	2017-10-10
AnnouncementXML	Submission_2017-10-26_04:42:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Tae Kim
SpeciesList	scientific name: Glycine max (Soybean) (Glycine hispida); NCBI TaxID: 3847;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-09 01:49:35	ID requested
1	2017-10-10 02:38:39	announced
⏵ 2	2017-10-26 04:42:19	announced	Updated publication reference for PubMed record(s): 29062869, 28669816.

Min CW, Lee SH, Cheon YE, Han WY, Ko JM, Kang HW, Kim YC, Agrawal GK, Rakwal R, Gupta R, Kim ST, seeds. Data Brief, 15():449-453(2017) [pubmed]

Min CW, Lee SH, Cheon YE, Han WY, Ko JM, Kang HW, Kim YC, Agrawal GK, Rakwal R, Gupta R, Kim ST, In-depth proteomic analysis of Glycine max seeds during controlled deterioration treatment reveals a shift in seed metabolism. J Proteomics, 169():125-135(2017) [pubmed]

ProteomeXchange project tag: Food and Nutrition (B/D-HPP)

curator keyword: Biological

submitter keyword: CDT, Quantitative proteomics, Low-abundance proteins, Abundant protein depletion, Soybean, Metabolic engineering

Sun Tae Kim
contact affiliation	Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, 627-706, South Korea
contact email	stkim5505@gmail.com
lab head
Sun Tae Kim
contact affiliation	Pusan National University
contact email	stkim71@pusan.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006064
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Glycine max seeds during controlled deterioration treatment