PXD006062

PXD006062 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Chinese Hamster Ovary cell proteins co-purified with recombinant HIV-1 envelopes, part 3
Description	Chinese Hamster Ovary (CHO) cells are frequently used to produce recombinant HIV envelope protein gp120. In this study, we characterized the CHO proteins that become co-purified with gp120 when lectin affinity chromatography is used. Many of co-purified CHO proteins identified were extracellular matrix components. We verified a method for separating gp120 from these CHO proteins with anion exchange chromatography, and assessed the biochemical activity of gp120 preparations with and without co-purified CHO proteins.
HostingRepository	PRIDE
AnnounceDate	2017-09-04
AnnouncementXML	Submission_2017-09-04_00:03:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Plotnik
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-09 01:35:52	ID requested
⏵ 1	2017-09-04 00:03:52	announced

Publication List

Plotnik D, Guo W, Cleveland B, von Haller P, Eng JK, Guttman M, Lee KK, Arthos J, Hu SL, . J Virol, 91(21):(2017) [pubmed]

Plotnik D, Guo W, Cleveland B, von Haller P, Eng JK, Guttman M, Lee KK, Arthos J, Hu SL, . J Virol, 91(21):(2017) [pubmed]

Keyword List

curator keyword: Technical, Biomedical
submitter keyword: HIV, gp120, fibronectin, extracellular matrix, CHO, Chinese Hamster

curator keyword: Technical, Biomedical

submitter keyword: HIV, gp120, fibronectin, extracellular matrix, CHO, Chinese Hamster

Contact List

Shiu-Lok Hu
contact affiliation	University of Washington
contact email	hus@wanprc.org
lab head
David Plotnik
contact affiliation	University of Washington
contact email	dplotnik@uw.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD006062
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD006062

PRIDE project URI

Repository Record List

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