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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-09-13
  • AnnouncementXML: Submission_2017-09-13_04:46:07.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Jacek Sikora
  • Title: Mitochondrial respiratory chain is essential for hematopoietic stem cell function
  • Description: Adult and fetal hematopoietic stem cells (HSCs) display a glycolytic phenotype, which is required for maintenance of stemness; however, whether mitochondrial respiration is required to maintain HSC function is not known. Here we report that loss of the mitochondrial complex III subunit Rieske iron sulfur protein (RISP) in fetal mouse HSCs allows them to proliferate but impairs their differentiation, resulting in anemia and prenatal death. RISP null fetal HSCs displayed impaired respiration resulting in a decreased NAD+/NADH ratio. RISP null fetal HSCs and progenitors exhibited an increase in both DNA and histone methylation concomitant with increases in 2-hydroxyglutarate (2-HG), a metabolite known to inhibit DNA and histone demethylases. RISP inactivation in adult HSCs also impaired respiration resulting in loss of quiescence resulting in severe pancytopenia and lethality. Thus, respiration is dispensable for adult or fetal HSC proliferation, but essential for fetal HSC differentiation and maintenance of adult HSC quiescence.
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
  • ModificationList: monomethylated residue; trimethylated residue; acetylated residue; dimethylated residue
  • Instrument: TSQ Quantum Ultra

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-03-08 06:05:10ID requested
12017-09-13 04:46:08announced

Publication List

  1. Ansó E, Weinberg SE, Diebold LP, Thompson BJ, Malinge S, Schumacker PT, Liu X, Zhang Y, Shao Z, Steadman M, Marsh KM, Xu J, Crispino JD, Chandel NS, The mitochondrial respiratory chain is essential for haematopoietic stem cell function. Nat Cell Biol, 19(6):614-625(2017) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Mitochondria, Metabolism, Stem Cell, HSC, Histone Modifications

Contact List

    Neil L. Kelleher
    • contact affiliation: Chemistry of Life Processes Institute, Proteomic Center of Excelence, Northwestern University, USA
    • contact email: n-kelleher@northwestern.edu
    • lab head:
    Jacek Sikora
    • contact affiliation: National Resource for Translational and Developmental Proteomics, Proteomics Center of Excellence, Northwestern University
    • contact email: j.sikora@northwestern.edu
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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