PXD006048

PXD006048 is an original dataset announced via ProteomeXchange.

Title	T. brucei MAPKLK1 knockdown
Description	Pathogenic trypanosomatids have a large number of protein kinases and phosphatases in comparison to other organisms. Moreover, post-translational modifications have key role in the gene expression control in these cells, reinforcing the relevance of the phosphorylation process. Nevertheless little is known about protein phosphorylation in these protozoa. In front of this, the effects of the depletion of a MAP kinase-like kinase (MAPKLK1) were evaluated in Trypanosoma brucei. After silencing MAPKLK1 expression by RNAi, the cells were evaluated by SILAC MS-based proteomics. In total, 1,756 phosphorylation sites were identified, of which 384 were not previously described in T. brucei. The modulations observed on proteome and phosphoproteome are related to key cellular processes enriched to mRNA processing and stability control.
HostingRepository	PRIDE
AnnounceDate	2017-10-17
AnnouncementXML	Submission_2017-10-17_03:08:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michel Batista
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	6x(13)C: 2x(15)N labeled L-lysine; phosphorylated residue; iodoacetamide derivatized residue; 6x(13)C labeled L-arginine
Instrument	LTQ Orbitrap XL ETD

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-08 01:45:08	ID requested
⏵ 1	2017-10-17 03:08:57	announced

Batista M, Kugeratski FG, de Paula Lima CV, Probst CM, Kessler RL, de Godoy LM, Krieger MA, Marchini FK, The MAP kinase MAPKLK1 is essential to Trypanosoma brucei proliferation and regulates proteins involved in mRNA metabolism. J Proteomics, 154():118-127(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Trypanosoma brucei, MAP kinase, LTQ Orbitrap XL ETD

Fabricio Klerynton Marchini
contact affiliation	Functional Genomics Laboratory, Carlos Chagas Institute, Fiocruz, Curitiba, Parana, Brazil
contact email	fabricio.marchini@fiocruz.br
lab head
Michel Batista
contact affiliation	Fiocruz
contact email	batista.mic@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD006048

PRIDE project URI

• PRIDE
  1. PXD006048
     1. Label: PRIDE project
     2. Name: T. brucei MAPKLK1 knockdown