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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-10-12
  • AnnouncementXML: Submission_2017-11-08_04:32:05.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: James Wright
  • Title: Cross-talk between PKA and PKG controls pH-dependent host cell egress of Toxoplasma gondii
  • Description: Toxoplasma gondii encodes three Protein Kinase A catalytic (PKAc1-3) and one regulatory (PKAr) subunits to integrate cAMP-dependent signals. Here, we show that inactive PKAc1 is maintained at the parasite pellicle by interacting with dually acylated PKAr. Either a conditional knockdown of PKAr or the overexpression of PKAc1 results in a block in parasite division. In contrast, conditional expression of a dominant negative PKAr isoform unable to bind cAMP, triggers premature egress of parasites from infected cells. This untimely egress critically depends on parasite density and host cell acidification. A comparative phosphoproteome analysis reveals that PKA genetic inhibition significantly changed the phosphorylation profile of a putative cGMP-phosphodiesterase, PDE2. Consistently, the phenotype of PKA genetic inhibition is alleviated by chemical inhibition of the cGMP-dependent protein kinase G (PKG). A phosphodiesterase inhibitor is able to circumvent egress repression by PKA or pH neutralisation, indicating that environmental acidification and PKA signalling act as balancing regulators of cGMP degradation to control PKG-mediated egress. Collectively, these results reveal a cross-talk between PKA and PKG pathways to govern egress in T. gondii.
  • SpeciesList: scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
  • ModificationList: phosphorylated residue; iodoacetamide derivatized residue; deamidated residue
  • Instrument: Orbitrap Fusion

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-03-07 07:51:49ID requested
12017-10-12 03:27:03announced
22017-11-08 04:32:06announcedUpdated publication reference for PubMed record(s): 29030485.

Publication List

  1. Jia Y, Marq JB, Bisio H, Jacot D, Mueller C, Yu L, Choudhary J, Brochet M, Soldati-Favre D, . EMBO J, 36(21):3250-3267(2017) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: LC-MSMS, IMAC, Phospho, T. gondii

Contact List

    Jyoti Choudhary
    • contact affiliation: Wellcome Trust Sanger Institute
    • contact email: jc4@sanger.ac.uk
    • lab head:
    James Wright
    • contact affiliation: Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute
    • contact email: jw13@sanger.ac.uk
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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