PXD006042

PXD006042 is an original dataset announced via ProteomeXchange.

Title	Affinity proteomics targeting the meiotic chromosome axis protein, ASY1, from Brassica oleracea.
Description	Meiosis is a highly complex process that underpins recombination in sexually reproducing organisms. Recent genomics studies suggest that up to several thousand genes/proteins contribute to the meiotic pathway, yet relatively few have been functionally characterised. Our understanding of the physical interactions between meiotic proteins and how the meiotic machinery interacts with other components of the cell is also limited. In this study, we used affinity proteomics targeting the meiotic chromosome axis protein, ASY1, to enrich for axis-associated proteins in Brassica oleracea anthers or meiocytes in prophase I of meiosis. LC-MS/MS analysis identified 540 proteins which co-immunoprecipitated with ASY1 in a sample-specific manner. These correspond to 485 Arabidopsis orthologues, 90% of which form a coherent predicted protein-protein interaction network which includes known and novel meiotic proteins, based on mutant analysis, but also proteins more usually associated with other cellular processes including replication and proteolysis. Our data provided new insights into the role of the chromosome axis in plant meiosis. We identified a novel axis-associated protein and showed that during prophase I, ASY1 and its interacting partner, ASY3, are extensively phosphorylated. Further studies targeting other meiotic proteins may ultimately enable the construction of a comprehensive meiosis protein-protein interaction network for higher plants.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:07:29.658.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Brassica oleracea var. alboglabra; NCBI TaxID: 3714;
ModificationList	methylthiolated residue; phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-07 03:48:48	ID requested
1	2017-11-01 02:34:46	announced
⏵ 2	2024-10-22 04:07:30	announced	2024-10-22: Updated project metadata.

10.1111/tpj.13752;

Osman K, Yang J, Roitinger E, Lambing C, Heckmann S, Howell E, Cuacos M, Imre R, D, ü, rnberger G, Mechtler K, Armstrong S, Franklin FCH, Affinity proteomics reveals extensive phosphorylation of the Brassica chromosome axis protein ASY1 and a network of associated proteins at prophase I of meiosis. Plant J, 93(1):17-33(2018) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, chromosome axis,Brassica, co-immunoprecipitation, protein-protein interaction, phosphorylation, meiosis, LC-MS/MS

F. Chris H. Franklin
contact affiliation	School of Biosciences, University of Birmingham, UK.
contact email	f.c.h.franklin@bham.ac.uk
lab head
Richard Imre
contact affiliation	IMBA - Institute of Molecular Biotechnology
contact email	imre@imp.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006042
     1. Label: PRIDE project
     2. Name: Affinity proteomics targeting the meiotic chromosome axis protein, ASY1, from Brassica oleracea.