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PXD006036

PXD006036 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMetabolic control of primed human pluripotent stem cell fate and function by the miR-200c-SIRT2 axis
DescriptionA hallmark of cancer cells is the metabolic switch from oxidative phosphorylation (OXPHOS) to glycolysis, a phenomenon referred to as the “Warburg effect”, which is also observed in primed human pluripotent stem cells (hPSCs). Here, we report that downregulation of SIRT2 and upregulation of SIRT1 is a molecular signature of primed hPSCs and that SIRT2 critically regulates metabolic reprogramming during induced pluripotency by targeting glycolytic enzymes including aldolase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, and enolase. Remarkably, knockdown of SIRT2 in human fibroblasts resulted in significantly decreased OXPHOS and increased glycolysis. In addition, we found that miR-200c-5p specifically targets SIRT2, downregulating its expression. Furthermore, SIRT2 overexpression in hPSCs significantly affected energy metabolism, altering stem cell functions such as pluripotent differentiation properties. Taken together, our results identify the miR-200c-SIRT2 axis as a key regulator of metabolic reprogramming (Warburg-like effect), via regulation of glycolytic enzymes, during human induced pluripotency and pluripotent stem cell function. To address our hypothesis that acetylation affects the metabolic switch, we compared protein acetylation in hESCs and hDFs by liquid chromatography-tandem mass spectrometry (LCMS/ MS) analyses following immunoprecipitation with acetyl-Lys antibody.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:07:18.971.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD006036
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterByung-gyu kim
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListPhospho; Deamidated; Oxidation; Acetyl; Carbamidomethyl
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-03-06 06:15:49ID requested
12018-10-26 09:17:45announced
22024-10-22 04:07:19announced2024-10-22: Updated project metadata.
Publication List
10.1038/ncb3517;
Cha Y, Han MJ, Cha HJ, Zoldan J, Burkart A, Jung JH, Jang Y, Kim CH, Jeong HC, Kim BG, Langer R, Kahn CR, Guarente L, Kim KS, Metabolic control of primed human pluripotent stem cell fate and function by the miR-200c-SIRT2 axis. Nat Cell Biol, 19(5):445-456(2017) [pubmed]
10.6019/PXD006036;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: acetylation, SIRT2, differentiation,human pluripotent stem cells
Contact List
Kwang-Soo Kim
contact affiliationMolecular Neurobiology Laboratory, Department of Psychiatry and McLean Hospital, Harvard Medical School
contact emailkskim@mclean.harvard.edu
lab head
Byung-gyu kim
contact affiliationCenter for genomic integrity (CGI), Institute of basic science (IBS), UNIST, Ulsan, 44919, Republic of Korea
contact emailgoldenlion@ibs.re.kr
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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