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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-07-17
  • AnnouncementXML: Submission_2017-07-17_04:29:39.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Roberta Noberini
  • Title: PAT-H-MS coupled with laser microdissection to study histone post-translational modifications in selected cell populations from pathology samples
  • Description: Aberrations in histone post-translational modifications (hPTMs) have been linked with various pathologies, including cancer, and could represent not only useful biomarkers, but also suggest possible targetable epigenetic mechanisms. We have recently developed an approach, termed pathology tissue analysis of histones by mass spectrometry (PAT-H-MS), that allows performing a comprehensive and quantitative analysis of histone H3 PTMs from formalin-fixed paraffin-embedded pathology samples. Despite its great potential, the application of this technique is limited by tissue heterogeneity. In this study, we implemented the PAT-H-MS approach by coupling it with techniques aimed at reducing sample heterogeneity and selecting specific portions or cell populations within the samples, such as manual macrodissection and lased micro-dissection (LMD). When applied to the analysis of a small set of breast cancer samples, LMD- PAT-H-MS allowed detecting more marked changes between Luminal A and Triple Negative patients as compared with the classical approach.
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: monomethylated residue; trimethylated residue; acetylated residue; dimethylated residue
  • Instrument: Q Exactive HF

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-03-06 01:53:29ID requested
12017-07-17 04:29:40announced

Publication List

  1. Noberini R, Longuespée R, Richichi C, Pruneri G, Kriegsmann M, Pelicci G, Bonaldi T, PAT-H-MS coupled with laser microdissection to study histone post-translational modifications in selected cell populations from pathology samples. Clin Epigenetics, 9():69(2017) [pubmed]

Keyword List

  1. curator keyword: Technical, Biomedical
  2. submitter keyword: histone post-translational modifications, PAT-H-MS, laser microdissection, proteomics, epigenetic marker, formalin-fixed paraffin embedded, mass spectrometry

Contact List

    Tiziana Bonaldi
    • contact affiliation: European Institute of Oncology, Italy
    • contact email: tiziana.bonaldi@ieo.it
    • lab head:
    Roberta Noberini
    • contact affiliation: Istituto Italiano di Tecnologia
    • contact email: roberta.noberini@iit.it
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
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