PXD006001

PXD006001 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide characterization of human S-acylated proteins
Description	Reversible protein S-acylation dynamically regulates the subcellular localization, activity, and stability of proteins, and plays a pivotal role in various diseases such as cancer and neurodegenerative disorders. Nevertheless, a system-wide analysis of S-acylation in human pathophysiology is still elusive, largely due to the lack of a sensitive method for proteome-wide characterization of S-acylation. Via systematic optimization of our original Palmitoyl-protein Identification and Site Characterization (PalmPISC) method, we established the next-generation PalmPISC (ngPalmPISC). The application of ngPalmPISC to human LNCaP cells identified 1,682 known and 1,175 novel candidate S-acylated proteins as well as 1,648 candidate S-acylation sites—a coverage of an order of magnitude larger than any previous studies. Moreover, the large set of S-acylation sites allowed motif enrichment analysis, resulting in the identification of two novel S-acylation motifs CxL and FxC (p<0.01). The ngPalmPISC method and the comprehensive human S-acylproteome dataset are expected to facilitate our system-wide understanding of protein S-acylation in pathophysiology and the discovery of novel biomarkers and therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2022-02-24
AnnouncementXML	Submission_2022-02-24_08:50:06.606.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	palmitoylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-02 01:06:38	ID requested
⏵ 1	2022-02-24 08:50:07	announced

Zhou B, Wang Y, Yan Y, Mariscal J, Di Vizio D, Freeman MR, Yang W, -Acylproteomic Analysis. Anal Chem, 91(15):9858-9866(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Palmitoylation, S-acylation, proteomics, LC-MS/MS, human, proteome-wide, motif, site

Wei Yang
contact affiliation	Departments of Surgery and Biomedical Sciences Cedars-Sinai Medical Center Los Angeles, CA 90048, USA
contact email	wei.yang@cshs.org
lab head
Wei Yang
contact affiliation	Cedars-Sinai Medical Center
contact email	wei.yang@cshs.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD006001

PRIDE project URI

• PRIDE
  1. PXD006001
     1. Label: PRIDE project
     2. Name: Proteome-wide characterization of human S-acylated proteins