PXD005995

PXD005995 is an original dataset announced via ProteomeXchange.

Title	Label-free differential proteomics analysis of Ba/F3 expressing WT and mutant R98S RPL10
Description	We recently identified a recurrent mutation in the ribosomal gene RPL10 that results in substitution of residue arginine 98 by a serine (R98S) of the ribosomal protein L10 (RPL10) in the contest of T-cell acute lymphoblastic leukemia (T-ALL). To gain insights into the mechanisms by which the RPL10 R98S mutation contributes to T-ALL development, we screened for proteins that are differentially expressed between RPL10 wild type (WT) and RPL10 R98S (R98S) expressing cells. These experiments were conducted in the mouse pro-B Ba/F3 cell line, a well-established hematopoietic model for oncogenic studies.
HostingRepository	PRIDE
AnnounceDate	2017-11-28
AnnouncementXML	Submission_2017-11-28_06:06:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Fancello
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-01 06:18:32	ID requested
⏵ 1	2017-11-28 06:06:45	announced

Girardi T, Vereecke S, Sulima SO, Khan Y, Fancello L, Briggs JW, Schwab C, de Beeck JO, Verbeeck J, Royaert J, Geerdens E, Vicente C, Bornschein S, Harrison CJ, Meijerink JP, Cools J, Dinman JD, Kampen KR, De Keersmaecker K, The T-cell leukemia-associated ribosomal RPL10 R98S mutation enhances JAK-STAT signaling. Leukemia, 32(3):809-819(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Leukemia, T-ALL, Ribosomal mutation, RPL10 R98S

Kim de Keersmaecker
contact affiliation	Department of Oncology, Laboratory for Disease mechanisms in cancer, KU Leuven Campus Gasthuisberg O&N1, Herestraat 49 - box 603 3000 Leuven (Belgium)
contact email	Kim.DeKeersmaecker@vib-kuleuven.be
lab head
Laura Fancello
contact affiliation	KU Leuven
contact email	laura.fancello@vib-kuleuven.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD005995

PRIDE project URI

• PRIDE
  1. PXD005995
     1. Label: PRIDE project
     2. Name: Label-free differential proteomics analysis of Ba/F3 expressing WT and mutant R98S RPL10