PXD005988

PXD005988 is an original dataset announced via ProteomeXchange.

Title	Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins
Description	The characterization of fish blood proteomes is important for the comparative studies of seminal and blood proteins as well as for the analysis of fish immune mechanisms and pathways. In this study LC-MS/MS and 2D DIGE was applied to compare seminal and blood plasma trout proteomes. The 54 differentially abundant proteins identified in seminal plasma are involved in a variety of signaling pathways, including protein ubiqutination, liver X receptor/retinoid X receptor (LXR/RXR) and farnesoid X (FXR)/RXR activation, cell cycle and acute phase signaling. These finding may indicate the prevalence of acute phase signaling pathways in trout seminal plasma, and its essential role in protecting spermatozoa and reproductive tissues. Furthermore our study provides the first in-depth analysis of the rainbow trout blood plasma proteome, with a total of 119 identified proteins. The major proteins of rainbow trout blood plasma were recognized as acute phase proteins. The Ingenuity Pathway Analysis of trout BP plasma proteins indicated that acute phase response signaling, complement system, LXR, FXR-RXR activation and coagulation system are the top canonical pathways associated with trout blood proteins. In conclusion this study enhances the knowledge of the blood origin of trout seminal plasma proteins and the understanding of fish reproductive biology. Additionally our results provide new insight into blood proteins specifically important for fish physiology and innate immunity.
HostingRepository	PRIDE
AnnounceDate	2017-04-11
AnnouncementXML	Submission_2017-04-11_07:57:51.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005988
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joanna Nynca
SpeciesList	scientific name: Oncorhynchus mykiss; NCBI TaxID: NCBITaxon:8022;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-28 07:55:59	ID requested
⏵ 1	2017-04-11 07:57:53	announced

Nynca J, Arnold G, Fr, ö, hlich T, Ciereszko A, Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins. Proteomics, 17(11):(2017) [pubmed]

curator keyword: Biological

submitter keyword: fish, blood plasma, proteome, mass spectrometry, innate immunity

Andrzej Ciereszko
contact affiliation	Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences
contact email	a.ciereszko@pan.olsztyn.pl
lab head
Joanna Nynca
contact affiliation	Department of Gamete and Embryo Biology
contact email	j.nynca@pan.olsztyn.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005988
     1. Label: PRIDE project
     2. Name: Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins