PXD005987

PXD005987 is an original dataset announced via ProteomeXchange.

Title	Characterisation of Myst2 and Niam interactomes in mouse embryonic stem cells
Description	Here we undertake the characterisation of Myst2 protein interactions in mouse embryonic stem cells by affinity purification and mass spectrometry using a Myst2-FTAP tagged cell line. This protein interaction study confirms that in mouse embryonic stem cells Myst2 is part of H3 and H4 histone acetylation complexes similar to those described in somatic cells. We identify a novel Myst2- associated protein, the tumour suppressor protein Niam (Nuclear Interactor of Arf and Mdm2), and show that it also interacts with subunits of Myst2 H4 HAT complexes. Human NIAM is involved in chromosome segregation, p53 regulation and cell proliferation in somatic cells, but its role in embryonic stem cells is unknown. We describe the first Niam embryonic stem cell interactome, which includes proteins with roles in DNA replication and repair, transcription, splicing and ribosome biogenesis. Many of Myst2 and Niam binding partners are required for correct embryonic development, implicating Myst2 and NIAM in the cooperative regulation of this process and suggesting a novel role for Niam in embryonic biology. The data provides a useful resource for exploring Myst2 and Niam essential cellular functions and should contribute to deeper understanding of organism development and survival as well as the development of cancer.
HostingRepository	PRIDE
AnnounceDate	2017-08-22
AnnouncementXML	Submission_2017-08-22_05:41:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Wright
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	2-pyrrolidone-5-carboxylic acid (Glu); monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ FT; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-28 05:03:41	ID requested
⏵ 1	2017-08-22 05:41:34	announced

Pardo M, Yu L, Shen S, Tate P, Bode D, Letney BL, Quelle DE, Skarnes W, Choudhary JS, Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells. Sci Rep, 7(1):8157(2017) [pubmed]

curator keyword: Biological

submitter keyword: blue native PAGE, Myst2, Niam, embryonic stem cells, protein interactions, affinity purification

Jyoti Choudhary
contact affiliation	Wellcome Trust Sanger Institute
contact email	jc4@sanger.ac.uk
lab head
James Wright
contact affiliation	Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute
contact email	jw13@sanger.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005987
     1. Label: PRIDE project
     2. Name: Characterisation of Myst2 and Niam interactomes in mouse embryonic stem cells