PXD005983
PXD005983 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Properties of the Cuticular Proteins of Anopheles Gambiae as Revealed by Serial Extraction of Adults |
| Description | How cuticular proteins (CPs) interact with chitin and with each other in the cuticle remains unresolved. We employed LC-MS/MS to identify CPs from 5-6 day-old adults of Anopheles gambiae released after serial extraction with PBS, EDTA, 2-8M urea, and SDS as well as those that remained unextracted. Results were compared to published data on time of transcript abundance, localization of proteins within structures and within the cuticle, as well as properties of individual proteins, length, pI, percent histidine, tyrosine, glutamine, and number of AAP[A/V/L] repeats. Thirteen proteins were solubilized completely, all were CPRs. Eleven were identified in both soluble fractions and the final pellet, including 5 from other CP families. Forty-four were only detected from the final pellet. These included CPRs and members of the CPAP1, CPF, CPFL, CPLCA, CPLCG, CPLCP, and TWDL families, as well as several low complexity CPs, not assigned to families. For a given protein, many histidines or tyrosines or glutamines appear to be potential participants in cross-linking since we could not identify consistent absence of individual peptides bearing these residues. We failed to recover peptides from the amino-terminus of any CP. Whether this implicates that location in sclerotization or some modification that prevents detection is not known. Soluble CPRs had lower isoelectric points than those that remained in the final pellet; most members of other CP families had isoelectric points of 8 or higher. |
| HostingRepository | MassIVE |
| AnnounceDate | 2017-02-28 |
| AnnouncementXML | Submission_2017-02-28_07:47:19.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Majors Badgett |
| SpeciesList | scientific name: Anopheles gambiae; common name: African malaria mosquito; NCBI TaxID: 7165; |
| ModificationList | Carbamidomethyl; Oxidation; Deamidated |
| Instrument | Orbitrap Fusion; LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2017-02-27 14:39:24 | ID requested | |
| ⏵ 1 | 2017-02-28 07:47:19 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: cuticular proteins, anopheles gambiae, Serial Extraction, mosquito, LC-MS/MS, chitin |
Contact List
| principal_investigator | |
|---|---|
| lab head | |
| Majors Badgett | |
| contact affiliation | University of Georgia |
| contact email | majorsbadgett@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000080580 |
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