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PXD005977

PXD005977 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMouse Hepatic Lipid Droplet Proteome under Fasting and Re-Feeding
DescriptionOne of the key functions of the mammalian liver is lipid metabolism. During fasting, lipid storage in the liver increases in order to reserve and provide energy for cellular functions. Upon re-feeding, this reserve of lipids is rapidly depleted; this change is visible, as the organelles responsible for lipid storage – lipid droplets (LDs) – drastically decrease in size following re-feeding. Little is known regarding LD proteome, or how it changes during the fasting/re-feeding transition. Our study investigated the hepatic LD proteome and how it changes between fasting and re-feeding conditions. For this purpose, LDs were isolated from 4 month-old C57BL/6 mice after a 24 hour fasting period, or a 24 hour fasting period followed by 6 hours of re-feeding. Proteins isolated from these LDs were subject to SDS-PAGE followed by in-gel trypsinization and LC-MS/MS. We identified a combined total of 941 proteins on hepatic LDs, of which 817 had quantifiable extracted ion chromatograms in at least 2 samples (n=6 total) and were not deemed contaminants. 777 of the 817 proteins were observed in both energetic states, with 33 being uniquely observed in fasted LDs, and 7 being uniquely observed in re-fed LDs.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:36:23.366.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDavid Kramer
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-02-27 03:44:55ID requested
12018-05-25 01:00:14announced
22018-05-29 08:54:30announcedUpdated publication reference for PubMed record(s): 29698803.
32024-10-22 04:36:31announced2024-10-22: Updated project metadata.
Publication List
Kramer DA, Quiroga AD, Lian J, Fahlman RP, Lehner R, Fasting and refeeding induces changes in the mouse hepatic lipid droplet proteome. J Proteomics, 181():213-224(2018) [pubmed]
10.1016/j.jprot.2018.04.024;
Keyword List
curator keyword: Biological
submitter keyword: Lipid Droplet Proteome,Mouse, Lipid metabolism, Liver LDs, Lipid Droplets, LC-MS/MS
Contact List
Richard Lehner
contact affiliationDepartment of Pediatrics, Group on Molecular and Cell Biology of Lipids 328 Heritage Medical Research Centre, University of Alberta, Edmonton, Alberta, Canada Phone: (780) 492-2963 Fax: (780) 492-3383 (fax)
contact emailrlehner@ualberta.ca
lab head
David Kramer
contact affiliationUniversity of Alberta Department of Biochemistry
contact emaildakramer@ualberta.ca
dataset submitter
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Dataset FTP location
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