PXD005977

PXD005977 is an original dataset announced via ProteomeXchange.

Title	Mouse Hepatic Lipid Droplet Proteome under Fasting and Re-Feeding
Description	One of the key functions of the mammalian liver is lipid metabolism. During fasting, lipid storage in the liver increases in order to reserve and provide energy for cellular functions. Upon re-feeding, this reserve of lipids is rapidly depleted; this change is visible, as the organelles responsible for lipid storage – lipid droplets (LDs) – drastically decrease in size following re-feeding. Little is known regarding LD proteome, or how it changes during the fasting/re-feeding transition. Our study investigated the hepatic LD proteome and how it changes between fasting and re-feeding conditions. For this purpose, LDs were isolated from 4 month-old C57BL/6 mice after a 24 hour fasting period, or a 24 hour fasting period followed by 6 hours of re-feeding. Proteins isolated from these LDs were subject to SDS-PAGE followed by in-gel trypsinization and LC-MS/MS. We identified a combined total of 941 proteins on hepatic LDs, of which 817 had quantifiable extracted ion chromatograms in at least 2 samples (n=6 total) and were not deemed contaminants. 777 of the 817 proteins were observed in both energetic states, with 33 being uniquely observed in fasted LDs, and 7 being uniquely observed in re-fed LDs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:36:23.366.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Kramer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-27 03:44:55	ID requested
1	2018-05-25 01:00:14	announced
2	2018-05-29 08:54:30	announced	Updated publication reference for PubMed record(s): 29698803.
⏵ 3	2024-10-22 04:36:31	announced	2024-10-22: Updated project metadata.

Kramer DA, Quiroga AD, Lian J, Fahlman RP, Lehner R, Fasting and refeeding induces changes in the mouse hepatic lipid droplet proteome. J Proteomics, 181():213-224(2018) [pubmed]

10.1016/j.jprot.2018.04.024;

curator keyword: Biological

submitter keyword: Lipid Droplet Proteome,Mouse, Lipid metabolism, Liver LDs, Lipid Droplets, LC-MS/MS

Richard Lehner
contact affiliation	Department of Pediatrics, Group on Molecular and Cell Biology of Lipids 328 Heritage Medical Research Centre, University of Alberta, Edmonton, Alberta, Canada Phone: (780) 492-2963 Fax: (780) 492-3383 (fax)
contact email	rlehner@ualberta.ca
lab head
David Kramer
contact affiliation	University of Alberta Department of Biochemistry
contact email	dakramer@ualberta.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD005977

PRIDE project URI

• PRIDE
  1. PXD005977
     1. Label: PRIDE project
     2. Name: Mouse Hepatic Lipid Droplet Proteome under Fasting and Re-Feeding