PXD005969

PXD005969 is an original dataset announced via ProteomeXchange.

Title	Evaluating the protein extraction methods for gut metaproteomics
Description	Human stool samples were collected, microbial cells were harvested and subjected to protein extraction using different protocols for metaproteomics analyses. The protein extraction methods differ from the usage of different lysis buffer (SDS, urea and commercially available B-Per reagent), bead beating, ultrasonication and heating. The impacts of different protein extraction methods on metaproteomics results were evaluated with multiple criteria including protein yields, peptide identifications, taxonomic compositions and functional compositions.
HostingRepository	PRIDE
AnnounceDate	2017-07-17
AnnouncementXML	Submission_2017-07-17_05:53:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xu Zhang
SpeciesList	scientific name: human gut metagenome; NCBI TaxID: 408170;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-27 01:47:00	ID requested
⏵ 1	2017-07-17 05:53:11	announced

Zhang X, Li L, Mayne J, Ning Z, Stintzi A, Figeys D, Assessing the impact of protein extraction methods for human gut metaproteomics. J Proteomics, 180():120-127(2018) [pubmed]

curator keyword: Technical

submitter keyword: metaproteomics, microbiome, protein extraction, bead beating, ultrasonication

Daniel Figeys
contact affiliation	BMI, Faculty of Medicine, University of Ottawa, Canada
contact email	dfigeys@uottawa.ca
lab head
Xu Zhang
contact affiliation	University of Ottawa
contact email	cheuzhang@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005969
     1. Label: PRIDE project
     2. Name: Evaluating the protein extraction methods for gut metaproteomics