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PXD005969

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-07-17
  • AnnouncementXML: Submission_2017-07-17_05:53:10.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Xu Zhang
  • Title: Evaluating the protein extraction methods for gut metaproteomics
  • Description: Human stool samples were collected, microbial cells were harvested and subjected to protein extraction using different protocols for metaproteomics analyses. The protein extraction methods differ from the usage of different lysis buffer (SDS, urea and commercially available B-Per reagent), bead beating, ultrasonication and heating. The impacts of different protein extraction methods on metaproteomics results were evaluated with multiple criteria including protein yields, peptide identifications, taxonomic compositions and functional compositions.
  • SpeciesList: scientific name: human gut metagenome; NCBI TaxID: 408170;
  • ModificationList: monohydroxylated residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-02-27 01:47:00ID requested
12017-07-17 05:53:11announced

Publication List

  1. Zhang X, Li L, Mayne J, Ning Z, Stintzi A, Figeys D, Assessing the impact of protein extraction methods for human gut metaproteomics. J Proteomics, ():(2017) [pubmed]

Keyword List

  1. curator keyword: Technical
  2. submitter keyword: metaproteomics, microbiome, protein extraction, bead beating, ultrasonication

Contact List

    Daniel Figeys
    • contact affiliation: BMI, Faculty of Medicine, University of Ottawa, Canada
    • contact email: dfigeys@uottawa.ca
    • lab head:
    Xu Zhang
    • contact affiliation: University of Ottawa
    • contact email: cheuzhang@gmail.com
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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