PXD005968

PXD005968 is an original dataset announced via ProteomeXchange.

Title	Prediction of protein complexes in Trypanosoma brucei by protein correlation profiling mass spectrometry and machine learning
Description	A disproportionate number of predicted proteins from the genome sequence of the protozoan parasite Trypanosoma brucei, an important human and animal pathogen, are hypothetical proteins of unknown function. This work describes a protein correlation profiling mass spectrometry approach, using two size exclusion and one ion exchange chromatography systems, to derive sets of predicted protein complexes in this organism by hierarchical clustering and machine learning methods. We provide examples of both potential new subunits of known protein complexes and of novel trypanosome complexes of suggested function, contributing to improving the functional annotation of the trypanosome proteome. These hypothesis-generating proteomic data are provided in an open access online data visualisation environment (http://134.36.66.166:8083/complex_explorer)
HostingRepository	PRIDE
AnnounceDate	2017-10-20
AnnouncementXML	Submission_2017-10-20_02:12:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Crozier
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-27 01:27:45	ID requested
⏵ 1	2017-10-20 02:12:27	announced

Crozier TWM, Tinti M, Larance M, Lamond AI, Ferguson MAJ, by Protein Correlation Profiling Mass Spectrometry and Machine Learning. Mol Cell Proteomics, 16(12):2254-2267(2017) [pubmed]

curator keyword: Biological

submitter keyword: Trypanosoma, procyclic, protein correlation profiling mass spectrometry, protein complexes

Angus Lamond
contact affiliation	Univeristy of Dundee
contact email	a.i.lamond@dundee.ac.uk
lab head
Thomas Crozier
contact affiliation	University of Cambridge
contact email	t.monteirocrozier@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005968
     1. Label: PRIDE project
     2. Name: Prediction of protein complexes in Trypanosoma brucei by protein correlation profiling mass spectrometry and machine learning