PXD005954 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Simple, scalable and sensitive tip-based identification of protease substrates |
Description | Large-scale N-terminomics using COFRADIC and TAILS has revolutionized protease research, but is disseminated only in few labs. We present an alternative and simple protocol for N-terminal peptide enrichment, based on charge-based fractional diagonal chromatography (ChaFRADIC) and requiring only well-established protein chemistry and a pipette tip. We achieve unprecedented sensitivity quantifying >2000 unique N-terminal peptides from 10 µg per sample, allowing the identification of proteolytic targets and consensus motifs. |
HostingRepository | PRIDE |
AnnounceDate | 2018-01-24 |
AnnouncementXML | Submission_2018-01-29_08:59:17.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laxmikanth Kollipara |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-02-23 01:16:22 | ID requested | |
1 | 2018-01-25 01:29:21 | announced | |
⏵ 2 | 2018-01-29 08:59:18 | announced | Updated publication reference for PubMed record(s): 29358340. |
Publication List
Shema G, Nguyen MTN, Solari FA, Loroch S, Venne AS, Kollipara L, Sickmann A, Verhelst SHL, Zahedi RP, Simple, scalable, and ultrasensitive tip-based identification of protease substrates. Mol Cell Proteomics, 17(4):826-834(2018) [pubmed] |
Keyword List
curator keyword: Technical |
submitter keyword: Human, SCX, LC-MS |
Contact List
Dr. René Peiman Zahedi |
contact affiliation | Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V. Otto-Hahn-Straße 6b 44227 Dortmund, Germany P: +49 (0)2 31.13 92-4143 F: +49 (0)2 31.13 92-4850 www.isas.de |
contact email | zahedi@isas.de |
lab head | |
Laxmikanth Kollipara |
contact affiliation | Leibniz – Institut für Analytische Wissenschaften - ISAS - e.V. |
contact email | laxmikanth.kollipara@isas.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD005954 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005954
- Label: PRIDE project
- Name: Simple, scalable and sensitive tip-based identification of protease substrates