PXD005954

PXD005954 is an original dataset announced via ProteomeXchange.

Title	Simple, scalable and sensitive tip-based identification of protease substrates
Description	Large-scale N-terminomics using COFRADIC and TAILS has revolutionized protease research, but is disseminated only in few labs. We present an alternative and simple protocol for N-terminal peptide enrichment, based on charge-based fractional diagonal chromatography (ChaFRADIC) and requiring only well-established protein chemistry and a pipette tip. We achieve unprecedented sensitivity quantifying >2000 unique N-terminal peptides from 10 µg per sample, allowing the identification of proteolytic targets and consensus motifs.
HostingRepository	PRIDE
AnnounceDate	2018-01-24
AnnouncementXML	Submission_2018-01-29_08:59:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laxmikanth Kollipara
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-23 01:16:22	ID requested
1	2018-01-25 01:29:21	announced
⏵ 2	2018-01-29 08:59:18	announced	Updated publication reference for PubMed record(s): 29358340.

Shema G, Nguyen MTN, Solari FA, Loroch S, Venne AS, Kollipara L, Sickmann A, Verhelst SHL, Zahedi RP, Simple, scalable, and ultrasensitive tip-based identification of protease substrates. Mol Cell Proteomics, 17(4):826-834(2018) [pubmed]

curator keyword: Technical

submitter keyword: Human, SCX, LC-MS

Dr. René Peiman Zahedi
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V. Otto-Hahn-Straße 6b 44227 Dortmund, Germany P: +49 (0)2 31.13 92-4143 F: +49 (0)2 31.13 92-4850 www.isas.de
contact email	zahedi@isas.de
lab head
Laxmikanth Kollipara
contact affiliation	Leibniz – Institut für Analytische Wissenschaften - ISAS - e.V.
contact email	laxmikanth.kollipara@isas.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD005954

PRIDE project URI

• PRIDE
  1. PXD005954
     1. Label: PRIDE project
     2. Name: Simple, scalable and sensitive tip-based identification of protease substrates