PXD005951

PXD005951 is an original dataset announced via ProteomeXchange.

Title	Screening for micropeptides in HUVEC without tryptic digestion, part 1
Description	Long non-coding RNAs (lncRNA) are transcribed but not translated ribonucleic acids with various functions. We analyzed a so far unreported lncRNA n342419, which we named MANTIS. A search for micropeptides after overexpression of MANTIS in Human Umbilical Vein Endothelial Cells (HUVEC) with subsequent LC-MS/MS without trypsination showed that none of the micropeptides found had any similarity to potential MANTIS ORFs, whereas the positive control yielded micropeptides encoded from GFP plasmid.
HostingRepository	PRIDE
AnnounceDate	2017-03-27
AnnouncementXML	Submission_2018-02-07_15:18:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-22 07:11:32	ID requested
1	2017-03-27 02:20:16	announced
⏵ 2	2018-02-07 15:18:08	announced	Updated publication reference for PubMed record(s): 29408197.

Josipovic I, Pfl, ü, ger B, Fork C, Vasconez AE, Oo JA, Hitzel J, Seredinski S, Gamen E, Heringdorf DMZ, Chen W, Looso M, Pullamsetti SS, Brandes RP, Leisegang MS, Long noncoding RNA LISPR1 is required for S1P signaling and endothelial cell function. J Mol Cell Cardiol, 116():57-68(2018) [pubmed]

curator keyword: Biological

submitter keyword: LC-MSMS, LncRNA, micropeptides

Ralf P. Brandes
contact affiliation	Institut für Kardiovaskuläre Physiologie, Goethe-Universität, Frankfurt am Main, Germany
contact email	brandes@vrc.uni-frankfurt.de
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD005951

PRIDE project URI

• PRIDE
  1. PXD005951
     1. Label: PRIDE project
     2. Name: Screening for micropeptides in HUVEC without tryptic digestion, part 1