PXD005943

PXD005943 is an original dataset announced via ProteomeXchange.

Title	Saccharomyces cerevisiae mRBPome in oxidative stress conditions
Description	This project aims to identify novel RNA binding proteins in the baker's yeast , Saccharomyces cerevisiae, involved in the oxidative stress,. Since interactions between RNAs and proteins may be transient, yeast cells, either untreated and growth in rich media and exposed to 0.5 mM hydrogen peroxide for 15 minutes were crosslinked with UV light at 254 nm which promotes the covalent link between proteins and RNAs. After this, polyadenylated mRNAs were purified via oligo(dT) coupled to magentic beads under stringent conditions. Finally, samples were subjected to mass spectrometry analysis. To rule out the possibility of RNA-independent binding we also analysed other samples where we performed competition assays with polyadenylic acid
HostingRepository	PRIDE
AnnounceDate	2021-06-10
AnnouncementXML	Submission_2022-03-14_04:26:22.644.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Matia Gonzalez
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-21 05:04:14	ID requested
1	2021-06-09 22:01:29	announced
⏵ 2	2022-03-14 04:26:23	announced	2022-03-14: Updated publication reference for PubMed record(s): 34278261.

Matia-Gonz, á, lez AM, Jabre I, Laing EE, Gerber AP, Oxidative stress induces coordinated remodeling of RNA-enzyme interactions. iScience, 24(7):102753(2021) [pubmed]

curator keyword: Biological

submitter keyword: Saccharomyces cerevisiae, RBP, LC-MS/MS, hydrogen peroxide, oxidative stress

Andre Paul Gerber
contact affiliation	Dept. of Microbial Sciences School of Biosciences and Medicine Faculty of Health and Medical Sciences University of Surrey Stag Hill Campus 19 AX 01 Guildford, GU2 7XH U.K.
contact email	a.gerber@surrey.ac.uk
lab head
Ana Matia Gonzalez
contact affiliation	University of Surrey
contact email	a.matia@surrey.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD005943

PRIDE project URI

• PRIDE
  1. PXD005943
     1. Label: PRIDE project
     2. Name: Saccharomyces cerevisiae mRBPome in oxidative stress conditions