PXD005932

PXD005932 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of intestinal mucosa from male mice lacking intestinal epithelial insulin receptors
Description	The goal of the present study was to determine whether loss of the insulin receptor alters the molecular landscape of the intestinal mucosa, using intestinal-epithelial insulin receptor knockout (IE-irKO) mice and both genetic (IRfl/fl and Villin-cre) controls. Quantitative proteomic analysis by Liquid Chromatography Mass Spectrometry (LC-MS) was deployed on jejunal and colonic mucosa from mice fed a chow- or Western diet (WD). Jejunal mucosa from IE-irKO mice demonstrated alterations in all intestinal cell linages, Paneth, goblet, absorptive and enteroendocrine cells, whereas only goblet and absorptive cells were affected in the colon. There was also a significant effect of the WD on the gut proteome. A significant reduction was detected in Paneth cell proteins with anti-microbial activity, including lysozyme C-1, angiogenin-4, cryptdin-related sequence1C-3 and -2, a-defensin 17 and intelectin-1a. The key protein expressed by goblet cells, mucin-2, was also reduced in the IE-irKO mice. Proteins involved in lipid metabolism, including aldose reductase-related protein 1, 15-hydroxyprostaglandin dehydrogenase [NAD(+)], apolipoprotein A-II and pyruvate dehydrogenase kinase isozyme 4, were increased in the mucosa of WD-fed IE-irKO mice as compared to controls. In contrast, expression of the nutrient-responsive gut hormones, glucose-dependent insulinotropic polypeptide and neurotensin, was reduced in the jejunal mucosa of IE-irKO mice, and there was a reduction in proteins of the P-type ATPases and the solute carrier-transporter family in the colon of WD-fed IE-irKO mice. In conclusion, IE-irKO mice display a distinct molecular phenotype, suggesting a biological role of insulin and its receptor in determining differentiated cell-specificity in the intestinal epithelium.
HostingRepository	PRIDE
AnnounceDate	2017-08-18
AnnouncementXML	Submission_2017-08-18_06:34:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Erwin Schoof
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-20 01:27:39	ID requested
⏵ 1	2017-08-18 06:34:30	announced

Jensen SR, Schoof EM, Wheeler SE, Hvid H, Ahnfelt-R, ø, nne J, Hansen BF, Nishimura E, Olsen GS, Kislinger T, Brubaker PL, Quantitative Proteomics of Intestinal Mucosa From Male Mice Lacking Intestinal Epithelial Insulin Receptors. Endocrinology, 158(8):2470-2485(2017) [pubmed]

curator keyword: Biological

submitter keyword: LC-MSMS, TMT, intestinal mucosa, InsR, in vivo

Patricia L. Brubaker
contact affiliation	Dept. of Physiology, University of Toronto, Toronto, ON, Canada Dept. of Medicine, University of Toronto, Toronto, ON, Canada
contact email	p.brubaker@utoronto.ca
lab head
Erwin Schoof
contact affiliation	University Health Network
contact email	eschoof@uhnresearch.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005932
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of intestinal mucosa from male mice lacking intestinal epithelial insulin receptors