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PXD005925

PXD005925 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentifications of protein kinase A substrates with quantitative phosphoproteomics and primary-sequence based scoring.(H-89)
DescriptionProtein kinase A (PKA) is a serine/threonine kinase who plays essential roles in the complex signalling pathways. To better understand the functionalities, it is necessary to elucidate direct interplays between PKA and their substrates in living system. In order to screen them in a high-throughput manner, we firstly quantified the change of phosphoproteome in the cells of which PKA activity was perturbed by drag stimulations. LC-MS/MS analysis identified 2,755 and 3,191 phosphopeptides from either experiments with activator or inhibitor of PKA. To exclude indirect targets of PKA, we built the simple model to characterize the kinase sequential specificity to its substrate recognitions based on the known kinase-substrate relationships. Finally, by applying the metric to the sites of which phosphorylation were changed due to the drug stimulations, we identified 29 reliable candidates of PKA targeting residues in living cells including 8 known substrates. Moreover, 18 sites out of them were confirmed to be site-specifically phosphorylated in vitro. Altogether this study proposed confident list of PKA substrate and expanded the knowledge of PKA signalling network.
HostingRepositoryjPOST
AnnounceDate2018-02-15
AnnouncementXMLSubmission_2022-09-18_02:59:28.594.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD005925
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTomoya Niinae
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex reporter+balance reagent N-acylated residue; L-methionine sulfoxide; TMT6plex reporter+balance reagent acylated N-terminal; S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-02-15 01:26:24ID requested
12018-02-14 07:00:06announced
22018-09-19 00:00:26announcedUpdated CV, FTP location and Modification list.
32019-08-20 22:54:55announcedUpdated PubMed.
42021-02-04 17:27:44announced2021-02-04: Updated FTP location.
52022-09-18 02:59:29announced2022-09-18: Updated FTP location.
Publication List
Imamura H, Wagih O, Niinae T, Sugiyama N, Beltrao P, Ishihama Y, Identifications of Putative PKA Substrates with Quantitative Phosphoproteomics and Primary-Sequence-Based Scoring. J Proteome Res, 16(4):1825-1830(2017) [pubmed]
Keyword List
submitter keyword: H-89, HeLa, TMT, PKA
Contact List
Yasushi Ishihama
lab head
Tomoya Niinae
contact affiliationKyoto university
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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