PXD005925
PXD005925 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identifications of protein kinase A substrates with quantitative phosphoproteomics and primary-sequence based scoring.(H-89) |
| Description | Protein kinase A (PKA) is a serine/threonine kinase who plays essential roles in the complex signalling pathways. To better understand the functionalities, it is necessary to elucidate direct interplays between PKA and their substrates in living system. In order to screen them in a high-throughput manner, we firstly quantified the change of phosphoproteome in the cells of which PKA activity was perturbed by drag stimulations. LC-MS/MS analysis identified 2,755 and 3,191 phosphopeptides from either experiments with activator or inhibitor of PKA. To exclude indirect targets of PKA, we built the simple model to characterize the kinase sequential specificity to its substrate recognitions based on the known kinase-substrate relationships. Finally, by applying the metric to the sites of which phosphorylation were changed due to the drug stimulations, we identified 29 reliable candidates of PKA targeting residues in living cells including 8 known substrates. Moreover, 18 sites out of them were confirmed to be site-specifically phosphorylated in vitro. Altogether this study proposed confident list of PKA substrate and expanded the knowledge of PKA signalling network. |
| HostingRepository | jPOST |
| AnnounceDate | 2018-02-15 |
| AnnouncementXML | Submission_2022-09-18_02:59:28.594.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD005925 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Tomoya Niinae |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | TMT6plex reporter+balance reagent N-acylated residue; L-methionine sulfoxide; TMT6plex reporter+balance reagent acylated N-terminal; S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2017-02-15 01:26:24 | ID requested | |
| 1 | 2018-02-14 07:00:06 | announced | |
| 2 | 2018-09-19 00:00:26 | announced | Updated CV, FTP location and Modification list. |
| 3 | 2019-08-20 22:54:55 | announced | Updated PubMed. |
| 4 | 2021-02-04 17:27:44 | announced | 2021-02-04: Updated FTP location. |
| ⏵ 5 | 2022-09-18 02:59:29 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Imamura H, Wagih O, Niinae T, Sugiyama N, Beltrao P, Ishihama Y, Identifications of Putative PKA Substrates with Quantitative Phosphoproteomics and Primary-Sequence-Based Scoring. J Proteome Res, 16(4):1825-1830(2017) [pubmed] |
Keyword List
| submitter keyword: H-89, HeLa, TMT, PKA |
Contact List
| Yasushi Ishihama | |
|---|---|
| lab head | |
| Tomoya Niinae | |
| contact affiliation | Kyoto university |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000234/ |
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