PXD005920

PXD005920 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of protein purified derivative of Mycobacterium bovis
Description	Tuberculin Skin Test (TST) based on in vivo intradermal inoculation of purified protein derivative from M. bovis (bPPD) is the diagnostic test for the control and surveillance of bovine tuberculosis (bTB). Proteomic analyses were performed on four bPPD samples from Mycobacterium bovis, strain AN5. Proteins were precipitated from bPPD solutions by TCA precipitation. The proteome of four bPPD preparations was investigated by bottom-up proteomics, which consisted in protein digestion and nano-LC-MS/MS analysis. Mass spectrometry analysis was performed on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer coupled online to an Easy nano-LC1000 system. This study represents the highest proteome coverage of bPPD preparations to date. Since proteins perform cellular functions essential to health and/or disease, obtaining knowledge of their presence and variance is of great importance in understanding disease states and for advancing translational studies. Therefore, to better understand Mycobacterium tuberculosis complex biology during infection, survival, and persistence, the reproducible evaluation of the proteins that catalyze and control these processes is critically important. More active and more specific tuberculins would be desirable. The cross-reactivity to non-tuberculous mycobacterium species (false-positive results) is crucial to develop better and more defined antigens.
HostingRepository	PRIDE
AnnounceDate	2017-04-05
AnnouncementXML	Submission_2017-04-05_03:52:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariaconcetta Varano
SpeciesList	scientific name: Mycobacterium bovis AN5; NCBI TaxID: 1389959;
ModificationList	4x(2)H labeled dimethylated residue; 2x(13)C: 6x(2)H labeled dimethylated L-arginine; monohydroxylated residue; alpha-amino dimethylated residue; N6: N6-dimethyl-L-lysine; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-14 04:02:45	ID requested
⏵ 1	2017-04-05 03:52:40	announced

Roperto S, Varano M, Russo V, Luc, à R, Cagiola M, Gaspari M, Ceccarelli DM, Cuda G, Roperto F, Proteomic analysis of protein purified derivative of Mycobacterium bovis. J Transl Med, 15(1):68(2017) [pubmed]

curator keyword: Biological

submitter keyword: PPD, M. bovis, Proteomics, Mass spectrometry

Franco Roperto
contact affiliation	Dipartimento di Biologia, Università di Napoli Federico II, Napoli, Italia
contact email	roperto@unina.it
lab head
Mariaconcetta Varano
contact affiliation	University of Catanzaro
contact email	mary.varano1@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD005920

PRIDE project URI

• PRIDE
  1. PXD005920
     1. Label: PRIDE project
     2. Name: Proteomic analysis of protein purified derivative of Mycobacterium bovis