PXD005919 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The CD24 receptor induces changes to the surface protein composition of B cell microvesicles with variable effects on their RNA and protein cargo |
Description | The CD24 cell surface receptor promotes apoptosis in developing B cells, and we recently found that it induces B cells to release plasma membrane-derived, CD24-bearing microvesicles (MVs). Here we have performed a systematic characterization of B cell MVs released from WEHI-231 B lymphoma cells in response to CD24 stimulation. We found that B cells constitutively release MVs of approximately 120 nm, and that CD24 induces an increase in phosphatidylserine-positive MV release. RNA cargo was predominantly comprised of 5S rRNA, regardless of stimulation; however, CD24 caused a decrease in the incorporation of protein coding transcripts. The MV proteome was enriched with mitochondrial and metabolism-related proteins after CD24 stimulation; however, these changes were variable and could not be fully validated by western blotting. CD24-bearing MVs carried Siglec-2, CD63, IgM, and, unexpectedly, Ter-119, but not Siglec-G or MHC-II despite their presence on the cell surface. CD24 stimulation also induced changes in CD63 and IgM expression on MVs not mirrored by the changes in cell surface expression. Overall, the composition of these MVs suggests that they may be involved in releasing mitochondrial components in response to pro-apoptotic stress with changes to the surface receptors potentially altering the cell type(s) that interact with the MVs. |
HostingRepository | PRIDE |
AnnounceDate | 2017-09-01 |
AnnouncementXML | Submission_2017-09-01_01:22:58.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andrew Joy |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-02-14 03:28:52 | ID requested | |
⏵ 1 | 2017-09-01 01:22:59 | announced | |
Publication List
Ayre DC, Chute IC, Joy AP, Barnett DA, Hogan AM, Gr, ü, ll MP, Pe, ñ, a-Castillo L, Lang AS, Lewis SM, Christian SL, CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo. Sci Rep, 7(1):8642(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: B lymphocyte, CD24, extracellular microvesicles, microvesicle, mitochondria, proteomics |
Contact List
Dr. Sherri Lynn Christian |
contact affiliation | Associate Professor, Dept. Biochemistry, Memorial University of Newfoundland, Canada |
contact email | sherri.christian@mun.ca |
lab head | |
Andrew Joy |
contact affiliation | Atlantic Cancer Research Institute |
contact email | andrewj@canceratl.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005919
- Label: PRIDE project
- Name: The CD24 receptor induces changes to the surface protein composition of B cell microvesicles with variable effects on their RNA and protein cargo