PXD005915

PXD005915 is an original dataset announced via ProteomeXchange.

Title	The costa of trichomonads: A complex macromolecular cytoskeleton structure made of uncommon proteins
Description	Background Information The costa is a prominent striated fiber that is found in protozoa of the Trichomonadidae family that present an undulating membrane. It is composed primarily of proteins that have not yet been explored. In this study, we used cell fractionation to obtain a highly enriched costa fraction whose structure and composition was further analyzed by electron microscopy and mass spectrometry. Results Electron microscopy of negatively stained samples revealed that the costa, which is a periodic structure with alternating electron-dense and electron-lucent bands, displays three distinct regions, named the head, neck and body. Fourier transform analysis showed that the electron-lucent bands present sub-bands with a regular pattern. An analysis of the costa fraction via one- and two-dimensional electrophoresis and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) allowed the identification of 54 hypothetical proteins. Fourteen of those proteins were considered to be major components of the fraction. Conclusions The costa of T. foetus is a complex and organized cytoskeleton structure made of a large number of proteins which is assembled into filamentous structures. Some of these proteins exhibit uncharacterized domains and no function related according to gene ontology, suggesting that the costa structure may be formed by a new class of proteins that differ from those previously described in other organisms. Seven of these proteins contain prefoldin domains displaying coiled-coil regions. This propriety is shared with proteins of the striated fibers of other protozoan as well as in intermediate filaments. Significance Our observations suggest the presence of a new class of the cytoskeleton filaments in T. foetus. We believe that our data could auxiliate in determine the specific locations of these proteins in the distinct regions that compose the costa, as well as to define the functional roles of each component. Therefore, our study will help in the better understanding of the organization and function of this structure in unicellular organisms.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:28:44.671.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eidy Santos
SpeciesList	scientific name: Tritrichomonas foetus; NCBI TaxID: 1144522;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-13 02:04:21	ID requested
1	2017-04-04 02:29:56	announced
⏵ 2	2024-10-22 04:28:48	announced	2024-10-22: Updated project metadata.

de Andrade Rosa I, Caruso MB, de Oliveira Santos E, Gonzaga L, Zingali RB, de Vasconcelos ATR, de Souza W, Benchimol M, The costa of trichomonads: A complex macromolecular cytoskeleton structure made of uncommon proteins. Biol Cell, 109(6):238-253(2017) [pubmed]

10.1111/boc.201600050;

submitter keyword: cytoskeleton, mass spectrometry, coiled-coils, Trichomonas vaginalis,Tritrichomonas foetus

Marlene Benchimol
contact affiliation	Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil and UNIGRANRIO-Universidade do Grande Rio, Caxias, Rio de Janeiro-Brazil
contact email	marlenebenchimol@gmail.com
lab head
Eidy Santos
contact affiliation	Universidade Estadual da Zona Oeste (UEZO), Rio de Janeiro, Brasil.
contact email	eidyos@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005915
     1. Label: PRIDE project
     2. Name: The costa of trichomonads: A complex macromolecular cytoskeleton structure made of uncommon proteins