PXD005900

PXD005900 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Phosphoprotoemics analysis of FAO cells stimulated with insulin
Description	FAO cells, rat hepatoma cells, were stimulated with 1 nM insulin and collected at seven time points for ~60 minutes. Ion metal affinity chromatography (IMAC) was performed to enrich the phosphopeptides. As we used two sets of 4-plex iTRAQ to quantify the peptides from seven samples, we set the internal control to calibrate two sets of phosphoproteomics data
HostingRepository	jPOST
AnnounceDate	2018-11-30
AnnouncementXML	Submission_2022-09-18_03:32:57.765.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Atsushi Hatano
SpeciesList	scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117;
ModificationList	S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; iTRAQ4plex reporter+balance reagent N-acylated residue; iTRAQ4plex reporter+balance reagent acylated N-terminal; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide
Instrument	QSTAR

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-09 21:06:26	ID requested
1	2018-11-29 07:00:14	announced
2	2021-02-04 16:44:26	announced	2021-02-04: Updated FTP location.
3	2021-12-20 20:22:21	announced	2021-12-21: Updated PubMed.
⏵ 4	2022-09-18 03:32:58	announced	2022-09-18: Updated FTP location.

Publication List

Yugi K, Kubota H, Toyoshima Y, Noguchi R, Kawata K, Komori Y, Uda S, Kunida K, Tomizawa Y, Funato Y, Miki H, Matsumoto M, Nakayama KI, Kashikura K, Endo K, Ikeda K, Soga T, Kuroda S, Reconstruction of insulin signal flow from phosphoproteome and metabolome data. Cell Rep, 8(4):1171-83(2014) [pubmed]

Yugi K, Kubota H, Toyoshima Y, Noguchi R, Kawata K, Komori Y, Uda S, Kunida K, Tomizawa Y, Funato Y, Miki H, Matsumoto M, Nakayama KI, Kashikura K, Endo K, Ikeda K, Soga T, Kuroda S, Reconstruction of insulin signal flow from phosphoproteome and metabolome data. Cell Rep, 8(4):1171-83(2014) [pubmed]

Keyword List

submitter keyword: insulin, rat hepatoma cell line

submitter keyword: insulin, rat hepatoma cell line

Contact List

Sinya Kuroda
lab head
Atsushi Hatano
contact affiliation	Department of Omics and Systems Biology, Niigata University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000233/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000233/