PXD005894

PXD005894 is an original dataset announced via ProteomeXchange.

Title	Proteome changes as consequence of altered c-di-GMP metabolism in BCG
Description	Pellicles, a type of biofilm, have gathered a renewed interest in the field of tuberculosis as a structure that mimics some characteristics occurring during M. tuberculosis infection, such as antibiotic recalcitrance and chronicity of infection. In other bacteria, it has been well documented that the second messenger c-di-GMP modulates the transition from planktonic cells to biofilm formation. In this work, we used two shotgun proteomics approaches, a label-free one to identify proteins present at two weeks of pellicle formation, and isobaric labeling to assess the differences in the proteome occurring among pellicles formed by three strains of M. bovis BCG: the parental strain (WT), a previously reported knock out in BCG1419c (∆PDE) gene and a new knock out in BCG1416c (∆DGC) gene, in order to unravel the role played by genes involved in the metabolism c-di-GMP during pellicle mode of growth. Our findings strongly suggest that in M. bovis BCG, c-di-GMP may positively correlate with enhanced tolerance to nitrosative stress and negatively to cell wall permeability, therefore suggesting hypothesis relevant for adaption to tolerance to several antibiotics.
HostingRepository	PRIDE
AnnounceDate	2018-03-21
AnnouncementXML	Submission_2018-03-21_01:53:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gustavo Diaz
SpeciesList	scientific name: Mycobacterium bovis BCG str. Pasteur 1173P2; NCBI TaxID: 410289;
ModificationList	TMT6plex reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-09 02:02:22	ID requested
⏵ 1	2018-03-21 01:53:32	announced

Segura-Cerda CA, Aceves-S, á, nchez MJ, Marquina-Castillo B, Mata-Espinoza D, Barrios-Pay, á, n J, Vega-Dom, í, nguez PJ, Pedroza-Rold, á, n C, Bravo-Madrigal J, Vallejo-Cardona AA, Hern, á, ndez-Pando R, Flores-Valdez MA, Immune response elicited by two rBCG strains devoid of genes involved in c-di-GMP metabolism affect protection versus challenge with M. tuberculosis strains of different virulence. Vaccine, 36(16):2069-2078(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Mycobacteria, pellicle, c-di-GMP, proteome

Nicole Kruh-Garcia
contact affiliation	Microbiology, Immunology and Pathology, Lab C210, Colorado State University, United States of America
contact email	Nicole.Kruh@colostate.edu
lab head
Gustavo Diaz
contact affiliation	Colorado State University
contact email	diaz.gustavo2011@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005894
     1. Label: PRIDE project
     2. Name: Proteome changes as consequence of altered c-di-GMP metabolism in BCG