PXD005893

PXD005893 is an original dataset announced via ProteomeXchange.

Title	Shotgun Proteomic Analysis of Proteins Present in Mosquito Ovaries at 24 and 72 h After a Blood Meal
Description	Female mosquitoes require a blood meal for oogenesis, and thereby receive a substantial iron load in the forms of holo-transferrin and hemoglobin. Our previous data showed that during digestion of a blood meal, the gut iron concentration decreases 10-fold, while that of the ovaries doubles from ingestion to 72 hours post feeding. Approximately 72 hours post feeding, eggs are laid with ~125 ng Fe each. We are interested in the effects of the blood meal on the expression of iron related proteins detected in the ovaries during time post feeding before eggs are laid. We have used shotgun proteomic analysis to identify proteins in the developing ovaries of Aedes aegypti; this information provides further insight into the effect of a blood meal on mosquito oogenesis.
HostingRepository	PRIDE
AnnounceDate	2022-03-26
AnnouncementXML	Submission_2022-03-25_17:16:02.041.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005893
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dawn Geiser
SpeciesList	scientific name: Aedes aegypti (Yellowfever mosquito) (Culex aegypti); NCBI TaxID: 7159;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-09 01:46:44	ID requested
⏵ 1	2022-03-25 17:16:03	announced

Geiser DL, Li W, Pham DQ, Wysocki VH, Winzerling JJ, Shotgun and TMT-Labeled Proteomic Analysis of the Ovarian Proteins of an Insect Vector, Aedes aegypti (Diptera: Culicidae). J Insect Sci, 22(2):(2022) [pubmed]

curator keyword: Biological

submitter keyword: Aedes aegypti, blood meal, eggs, ferritin, iron, ovary, proteomics

Joy J. Winzerling
contact affiliation	University of Arizona Professor Emerita, Department of Nutritional Science Associate Dean, Bart Cardon Career and Academic Services
contact email	jwinzerl@email.arizona.edu
lab head
Dawn Geiser
contact affiliation	University of Arizona
contact email	dlgeiser@email.arizona.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD005893

PRIDE project URI

• PRIDE
  1. PXD005893
     1. Label: PRIDE project
     2. Name: Shotgun Proteomic Analysis of Proteins Present in Mosquito Ovaries at 24 and 72 h After a Blood Meal