PXD005892

PXD005892 is an original dataset announced via ProteomeXchange.

Title	Investigating Reporter Ion Signal Characteristics in Isobaric Mass Tag Proteome Quantification Experiments
Description	In this study we sought to examine the characteristics of the reporter ion signal obtained using current generation OT-based MS hardware. We initially focus on re-analysis of a previous experiment where TMT was using in combination with OT-MS3 to study protein expression diversity between histotypes of ovarian carcinoma where the presence of a notch – a region with no reported values – was observed in the reporter ion signal distributions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:36:10.298.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Hughes
SpeciesList	scientific name: Ralstonia eutropha JMP134; NCBI TaxID: 264198; scientific name: Yarrowia lipolytica (Candida lipolytica); NCBI TaxID: 4952; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-09 01:41:56	ID requested
1	2017-05-02 05:02:44	announced
⏵ 2	2024-10-22 04:36:11	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.7b00092;

Hughes CS, Zhu C, Spicer V, Krokhin OV, Morin GB, Evaluating the Characteristics of Reporter Ion Signal Acquired in the Orbitrap Analyzer for Isobaric Mass Tag Proteome Quantification Experiments. J Proteome Res, 16(5):1831-1838(2017) [pubmed]

curator keyword: Technical

submitter keyword: MS3,TMT, Orbitrap Fusion

Gregg Morin
contact affiliation	Head of Proteomics
contact email	gmorin@bcgsc.ca
lab head
Christopher Hughes
contact affiliation	BC Cancer Agency
contact email	chughes@bcgsc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD005892

PRIDE project URI

• PRIDE
  1. PXD005892
     1. Label: PRIDE project
     2. Name: Investigating Reporter Ion Signal Characteristics in Isobaric Mass Tag Proteome Quantification Experiments