PXD005890

PXD005890 is an original dataset announced via ProteomeXchange.

Title	Improving Mass Spectrometer Acquisition when using Tandem MS/MS/MS Scanning with Isobaric Tags
Description	n this work we utilize a standard, high-complexity Human cell line tryptic digest combined with a synthetic peptide library to systematically evaluate the properties of a variety of MS operation modes and detector configurations for the optimal examination of isobaric-tagged peptide mixtures. We demonstrate that despite the ability of the IT to yield more rapid MS3 scanning, the benefit in peptide and protein identifications when examining complex mixtures is minor while the negative impact on quantification precision is significant.
HostingRepository	PRIDE
AnnounceDate	2017-04-26
AnnouncementXML	Submission_2017-04-26_04:50:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Hughes
SpeciesList	scientific name: Ralstonia eutropha JMP134; NCBI TaxID: 264198; scientific name: Yarrowia lipolytica (Candida lipolytica); NCBI TaxID: 4952; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-09 01:36:43	ID requested
⏵ 1	2017-04-26 04:50:55	announced

Hughes CS, Spicer V, Krokhin OV, Morin GB, Investigating Acquisition Performance on the Orbitrap Fusion When Using Tandem MS/MS/MS Scanning with Isobaric Tags. J Proteome Res, 16(5):1839-1846(2017) [pubmed]

curator keyword: Technical

submitter keyword: TMT, MS3, Orbitrap Fusion

Gregg Morin
contact affiliation	Head of Proteomics
contact email	gmorin@bcgsc.ca
lab head
Christopher Hughes
contact affiliation	British Columbia Cancer Research Centre
contact email	chughes@bcgsc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD005890

PRIDE project URI

• PRIDE
  1. PXD005890
     1. Label: PRIDE project
     2. Name: Improving Mass Spectrometer Acquisition when using Tandem MS/MS/MS Scanning with Isobaric Tags