PXD005883

PXD005883 is an original dataset announced via ProteomeXchange.

Title	Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation
Description	The human cytomegalovirus (HCMV) US12 family consists of ten sequentially arranged genes (US12-21) with poorly characterized function. We now identify novel NK cell evasion functions for four members: US12, US14, US18 and US20. Using a systematic multiplexed proteomics approach to quantify ~1,300 cell surface and ~7,200 whole cell proteins, we demonstrate that the US12 family selectively targets plasma membrane proteins and plays key roles in regulating NK ligands, adhesion molecules and cytokine receptors. US18 and US20 work in concert to suppress cell surface expression of the critical NKp30 ligand B7-H6 thus inhibiting NK cell activation. The US12 family is therefore identified as a major new hub of immune regulation.
HostingRepository	PRIDE
AnnounceDate	2017-02-14
AnnouncementXML	Submission_2017-02-14_07:33:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yagnesh Umrania
SpeciesList	scientific name: Human herpesvirus 5; NCBI TaxID: 10359; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; carbamoylated residue; monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-08 06:10:03	ID requested
⏵ 1	2017-02-14 07:33:26	announced

Fielding CA, Weekes MP, Nobre LV, Ruckova E, Wilkie GS, Paulo JA, Chang C, Su, á, rez NM, Davies JA, Antrobus R, Stanton RJ, Aicheler RJ, Nichols H, Vojtesek B, Trowsdale J, Davison AJ, Gygi SP, Tomasec P, Lehner PJ, Wilkinson GW, Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation. Elife, 6():(2017) [pubmed]

submitter keyword: human cytomegalovirus, natural killer, multiplexed proteomics, cell surface

Michael Weekes
contact affiliation	Cambridge Institute for Medical Research, Hills Road, Cambridge, CB2 0XY
contact email	mpw1001@cam.ac.uk
lab head
Yagnesh Umrania
contact affiliation	University of Cambridge
contact email	yu206@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005883

PRIDE project URI

• PRIDE
  1. PXD005883
     1. Label: PRIDE project
     2. Name: Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation