PXD005873

PXD005873 is an original dataset announced via ProteomeXchange.

Title	Systemic perturbation of keratinocyte homeostasis
Description	We performed comprehensive proteome profiling (ECM and cell lysate) of human collagen VII deficient and control epidermal keratinocytes to generate global and detailed images of dysregulated epidermal molecular pathways linked to loss of collagen VII. These revealed downregulation of interaction partners of collagen VII, but also increased abundance of S100 pro-inflammatory proteins and enhanced activity of lysosomal proteases. Thus, loss of a single extracellular structural protein, collagen VII, induced persistent fibrosis enabling inflammatory and tissue destabilizing activities of keratinocytes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:36:10.996.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joern Dengjel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-08 00:00:11	ID requested
1	2018-01-16 02:26:45	announced
2	2020-04-21 22:34:15	announced	2020-04-22: Updated publication reference for PubMed record(s): 32305317, 29326176.
⏵ 3	2024-10-22 04:36:26	announced	2024-10-22: Updated project metadata.

10.1016/j.jid.2020.02.040;

10.1074/mcp.ra117.000437;

Berberich B, Thriene K, Gretzmeier C, K, ü, hl T, Bayer H, Athanasiou I, Rafei-Shamsabadi DA, Bruckner-Tuderman L, Nystr, ö, m A, Kiritsi D, Dengjel J, Proteomic Profiling of Fibroblasts Isolated from Chronic Wounds Identifies Disease-Relevant Signaling Pathways. J Invest Dermatol, 140(11):2280-2290.e4(2020) [pubmed]

Thriene K, Gr, ü, ning BA, Bornert O, Erxleben A, Leppert J, Athanasiou I, Weber E, Kiritsi D, Nystr, ö, m A, Reinheckel T, Backofen R, Has C, Bruckner-Tuderman L, Dengjel J, Combinatorial Omics Analysis Reveals Perturbed Lysosomal Homeostasis in Collagen VII-deficient Keratinocytes. Mol Cell Proteomics, 17(4):565-579(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: ECM, keratinocyte, matrix, LC-MS, SILAC, collagen,human

Joern Dengjel
contact affiliation	Dep. of Biology, University of Fribourg, Switzerland
contact email	joern.dengjel@unifr.ch
lab head
Joern Dengjel
contact affiliation	University of Fribourg
contact email	joern.dengjel@medizin.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD005873

PRIDE project URI

• PRIDE
  1. PXD005873
     1. Label: PRIDE project
     2. Name: Systemic perturbation of keratinocyte homeostasis