PXD005870

PXD005870 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of Sf21 cells during Baculovirus infection reveals progressive host proteome changes and its regulation by viral miRNA.
Description	System level knowledge of host alterations is crucial to elucidate the molecular events of viral pathogenesis and to develop strategies to block viral establishment and amplification. Here, we applied quantitative proteomics approach to study global proteome changes in the host; Spodoptera frugiperda upon infection by a baculovirus, Spodoptera litura NPV at two stages i.e. 12h and 72h post infection. At 12hpi, >95% of host proteins remained stable, however at 72hpi, 52% host proteins exhibited downregulation of 2-fold or more. Functional analysis revealed significant upregulation of transposition and proteasomal machinery while translation, transcription, protein export and oxidative phosphorylation pathways were adversely affected. An assessment of perturbed proteome after viral infection and viral miRNAs expression led to the identification of 117 genes that are potential targets of 10 viral miRNAs. Using miRNA mimics, we confirmed the down regulation of 9 host genes. The results comprehensively show dynamics of host responses after viral infection.
HostingRepository	PRIDE
AnnounceDate	2017-09-25
AnnouncementXML	Submission_2017-09-25_02:00:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dinesh Gupta
SpeciesList	scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos; LCQ Classic

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-07 10:39:43	ID requested
⏵ 1	2017-09-25 02:00:35	announced

Nayyar N, Kaur I, Malhotra P, Bhatnagar RK, Quantitative proteomics of Sf21 cells during Baculovirus infection reveals progressive host proteome changes and its regulation by viral miRNA. Sci Rep, 7(1):10902(2017) [pubmed]

curator keyword: Biological

submitter keyword: insect cell, baculovirus, proteomics, miRNA

Dr. Raj Bhatnagar
contact affiliation	International Centre for Genetic engineering and Biotechnology, New Delhi, India
contact email	raj@icgeb.res.in
lab head
Dinesh Gupta
contact affiliation	ICGEB
contact email	dinesh@icgeb.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD005870

PRIDE project URI

• PRIDE
  1. PXD005870
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of Sf21 cells during Baculovirus infection reveals progressive host proteome changes and its regulation by viral miRNA.