PXD005863

PXD005863 is an original dataset announced via ProteomeXchange.

Title	Identification of TH2B partners
Description	Histone replacement by transition proteins (TPs) and protamines (Prms) constitutes an essential step for the successful production of functional male gametes, yet nothing is known on the underlying functional interplay between histones, TPs and Prms. Here, by studying spermatogenesis in the absence of H2A.L.2, a spermatid-specific histone variant, we identify a new step, which is essential for the transformation of nucleosomes to nucleoprotamines. H2A.L.2, synthesized at the same time as TPs, enables the invasion of nucleosomes by TPs, which themselves drive the recruitment and processing of Prms. H2A.L.2 incorporation initiates a series of successive transitional states that ultimately shift to a more stable state characterized by nucleoprotamine assembly and histone-TP displacement, leading to the generation of mature spermatozoa. Hence, the current view of histone-to-nucleoprotein transition should be revisited and include a new step with H2A.L.2 assembly prior to the action of TPs and Prms.
HostingRepository	PRIDE
AnnounceDate	2017-04-24
AnnouncementXML	Submission_2018-01-30_08:45:19.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005863
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-07 04:22:20	ID requested
1	2017-04-24 08:05:43	announced
⏵ 2	2018-01-30 08:45:20	announced	Updated publication reference for PubMed record(s): 28366643.

Barral S, Morozumi Y, Tanaka H, Montellier E, Govin J, de Dieuleveult M, Charbonnier G, Cout, é Y, Puthier D, Buchou T, Boussouar F, Urahama T, Fenaille F, Curtet S, H, é, ry P, Fernandez-Nunez N, Shiota H, G, é, rard M, Rousseaux S, Kurumizaka H, Khochbin S, Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells. Mol Cell, 66(1):89-101.e8(2017) [pubmed]

curator keyword: Biological

submitter keyword: TH2B, co-immunoprecipitation, nanoLC-MS/MS

Virginie Brun
contact affiliation	EDyP
contact email	virginie.brun@cea.fr
lab head
Yohann Couté
contact affiliation	Laboratoire d'Etude de la Dynamique des Protéomes
contact email	yohann.coute@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD005863

PRIDE project URI

• PRIDE
  1. PXD005863
     1. Label: PRIDE project
     2. Name: Identification of TH2B partners