PXD005846

PXD005846 is an original dataset announced via ProteomeXchange.

Title	Cell surface mapping and monitoring of cell surface dynamics
Description	Here, we present a simple and robust N-linked glycoproteome enrichment protocol optimized for deep coverage of the plasma membrane proteome. The procedure was applied to characterize the cell surface proteome of 15 standard laboratory human cell lines and three primary cell types. We observed significant differences in receptor diversity and transporter abundances between primary cells and corresponding cell lines. Relative quantification using isobaric mass tagging enabled quantitative monitoring of dynamic processes on the cell surface in multiplexed experiments. We for the first time report a comprehensive analysis of the profound remodeling of the plasma membrane proteome during monocyte to macrophage differentiation of THP-1 cells. Treatment of these cells with the kinase inhibitor dasatinib (Sprycel) during differentiation severely compromised macrophage differentiation due to an off-target activity resulting in substantial morphological and functional changes and the loss of many macrophage-associated proteins.
HostingRepository	PRIDE
AnnounceDate	2017-03-27
AnnouncementXML	Submission_2017-03-27_08:42:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Faelth Savitski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-04 05:59:46	ID requested
⏵ 1	2017-03-27 08:42:20	announced

Kalxdorf M, Gade S, Eberl HC, Bantscheff M, -Glycoproteome Dynamics by Quantitative Proteomics Reveals Mechanistic Insights into Macrophage Differentiation. Mol Cell Proteomics, 16(5):770-785(2017) [pubmed]

curator keyword: Technical, Biological, Biomedical

submitter keyword: N-linked glycoproteomics, cell surface, primary cells

Christian Eberl
contact affiliation	Cellzome, a GSK company
contact email	hans-christian.h.eberl@gsk.com
lab head
Maria Faelth Savitski
contact affiliation	Cellzome
contact email	cz.pride@gsk.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD005846

PRIDE project URI

• PRIDE
  1. PXD005846
     1. Label: PRIDE project
     2. Name: Cell surface mapping and monitoring of cell surface dynamics