PXD005843 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Zebrafish retinal proteome during experimental degeneration and regeneration |
| Description | Zebrafish spontaneously regenerate the retina after injury. Currently, studies have observed gene expression profiles in this species however this may be a poor reflection of protein function. To further address this and expand our understanding of the regenerative process in the zebrafish, we compared the proteomic profile of the retina during injury and upon regeneration. Ouabain was injected intravitreously to create a model of degeneration and regeneration of the retina which was extracted at 0, 3 and 18 days after injection. Differences in protein expression indicates reduced metabolic processing, and increase in fibrin clot formation, with significant upregulation of fibrinogen gamma polypeptide, apolipoproteins A-Ib and A-II, galectin-1, and vitellogenin-6 during degeneration when compared to normal retina. In addition, cytoskeleton and membrane transport proteins were considerably altered during regeneration, with the highest fold upregulation observed for tubulin beta 2A, histone H2B and brain type fatty acid binding protein. Key proteins identified in this study may play an important role in the regeneration of the zebrafish retina and investigations on the potential regulation of these proteins may support future investigations in this field. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_04:35:33.744.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD005843 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Karen Eastlake |
| SpeciesList | scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q-Tof Premier |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2017-02-03 06:10:32 | ID requested | |
| 1 | 2017-03-21 09:38:25 | announced | |
| ⏵ 2 | 2024-10-22 04:35:39 | announced | 2024-10-22: Updated project metadata. |
Publication List
| Eastlake K, Heywood WE, Tracey-White D, Aquino E, Bliss E, Vasta GR, Mills K, Khaw PT, Moosajee M, Limb GA, Comparison of proteomic profiles in the zebrafish retina during experimental degeneration and regeneration. Sci Rep, 7():44601(2017) [pubmed] |
| 10.6019/PXD005843; |
| 10.1038/srep44601; |
Keyword List
| curator keyword: Biological |
| submitter keyword: degeneration, regeneration,Zebrafish, retina |
Contact List
| G. Astrid Limb |
|---|
| contact affiliation | National Institute for Health Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, London, EC1V 9EL |
| contact email | g.limb@ucl.ac.uk |
| lab head | |
| Karen Eastlake |
|---|
| contact affiliation | University College London |
| contact email | k.eastlake@ucl.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD005843 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005843
- Label: PRIDE project
- Name: Zebrafish retinal proteome during experimental degeneration and regeneration
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