PXD005831

PXD005831 is an original dataset announced via ProteomeXchange.

Title	Concerted regulation of ISWI by an autoinhibitory domain and the H4 N-terminal tail
Description	ISWI-family nucleosome remodeling enzymes need the histone H4 N-terminal tail to mobilize nucleosomes. Here we mapped the H4-tail binding pocket of ISWI. Surprisingly the binding site was adjacent to but not overlapping with the docking site of an auto-regulatory motif, AutoN, in the N-terminal region (NTR) of ISWI, indicating that AutoN does not act as a simple pseudosubstrate as suggested previously. Rather, AutoN cooperated with a hitherto uncharacterized motif, termed AcidicN, to confer H4-tail sensitivity and discriminate between DNA and nucleosomes. A third motif in the NTR, ppHSA, was functionally required in vivo and provided structural stability by clamping the NTR to Lobe 2 of the ATPase domain. This configuration is reminiscent of Chd1 even though Chd1 contains an unrelated NTR. Our results shed light on the intricate structural and functional regulation of ISWI by the NTR and uncover surprising parallels with Chd1. Elife. 2017 Jan 21;6. pii: e21477. doi: 10.7554/eLife.21477. [Epub ahead of print]
HostingRepository	PRIDE
AnnounceDate	2017-02-02
AnnouncementXML	Submission_2017-02-02_05:03:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johanna Ludwigsen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-01 07:01:42	ID requested
⏵ 1	2017-02-02 05:03:49	announced

Ludwigsen J, Pfennig S, Singh AK, Schindler C, Harrer N, Forn, é I, Zacharias M, Mueller-Planitz F, Concerted regulation of ISWI by an autoinhibitory domain and the H4 N-terminal tail. Elife, 6():(2017) [pubmed]

curator keyword: Biological

submitter keyword: ISWI, SNF2H, Chd1, crosslinking

Felix Mueller-Planitz
contact affiliation	BioMedizinisches Centrum - LMU Molecular Biology Großhaderner Str. 9 82152 Planegg-Martinsried GERMANY
contact email	Felix.Mueller-Planitz@med.uni-muenchen.de
lab head
Johanna Ludwigsen
contact affiliation	Ludwig-Maximilians University Munich
contact email	Johanna.Ludwigsen@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005831

PRIDE project URI

• PRIDE
  1. PXD005831
     1. Label: PRIDE project
     2. Name: Concerted regulation of ISWI by an autoinhibitory domain and the H4 N-terminal tail