PXD005823

PXD005823 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comparative proteomic analysis and bioluminescent reporter gene assays to investigate effects of simulated microgravity on Caco-2 cells
Description	Microgravity is one of the most important features in spaceflight. Previous evidence has shown that significant changes to the musculoskeletal and immune systems occurred under microgravity. The present study was undertaken to explore the change in protein abundance in human colon colorectal cells that were incubated for 48 or 72 h either in normal conditions and µG simulated conditions. The comparative proteomic method based on the 18O labeling technique was applied to investigate the up-regulated proteins and down-regulated proteins in SH-SY5Y under simulated microgravity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:36:07.720.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Riccardo Zenezini Chiozzi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-01 01:18:26	ID requested
1	2017-07-26 04:15:40	announced
⏵ 2	2024-10-22 04:36:09	announced	2024-10-22: Updated project metadata.

Publication List

La Barbera G, Capriotti AL, Michelini E, Piovesana S, Calabretta MM, Zenezini Chiozzi R, Roda A, Lagan, à A, Proteomic analysis and bioluminescent reporter gene assays to investigate effects of simulated microgravity on Caco-2 cells. Proteomics, 17(15-16):(2017) [pubmed]
10.1002/pmic.201700081;

La Barbera G, Capriotti AL, Michelini E, Piovesana S, Calabretta MM, Zenezini Chiozzi R, Roda A, Lagan, à A, Proteomic analysis and bioluminescent reporter gene assays to investigate effects of simulated microgravity on Caco-2 cells. Proteomics, 17(15-16):(2017) [pubmed]

10.1002/pmic.201700081;

Keyword List

curator keyword: Biological
submitter keyword: simulated microgravity, Nuclear factor κ-B,Caco-2 cells, reporter gene technology, Label-free proteomics, bioluminescence

curator keyword: Biological

submitter keyword: simulated microgravity, Nuclear factor κ-B,Caco-2 cells, reporter gene technology, Label-free proteomics, bioluminescence

Contact List

Aldo Laganà
contact affiliation	1Dipartimento di Chimica, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy
contact email	aldo.lagana@uniroma1.it
lab head
Riccardo Zenezini Chiozzi
contact affiliation	university la Sapienza, Roma
contact email	riccardo.zenezini@uniroma1.it
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD005823
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD005823

PRIDE project URI

Repository Record List

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