PXD005812

PXD005812 is an original dataset announced via ProteomeXchange.

Title	Proteomics of FACS-sorted heterogeneous Corynebacterium glutamicum populations
Description	The metabolic status of individual cells in microbial cultures can differ being relevant for biotechnology, environmental and medical microbiology. However, it is hardly understood in molecular detail due to limitations of current analytical tools. Here, we demonstrate that FACS in combination with proteomics can be used to sort and analyze cell populations based on their metabolic state. A previously established GFP reporter system was used to detect and sort single Corynebacterium glutamicum cells based on the concentration of branched chain amino acids (BCAA) using FACS. A proteomics workflow optimized for small cell numbers was used to quantitatively compare proteomes of a ?aceE mutant, lacking functional pyruvate dehydrogenase (PD), and the wild type. About 800 proteins could be quantified from 1,000,000 cells. In the ?aceE mutant BCAA production was coordinated with upregulation of the glyoxylate cycle and TCA cycle to counter the lack of acetyl CoA resulting from the deletion of aceE.
HostingRepository	PRIDE
AnnounceDate	2017-11-02
AnnouncementXML	Submission_2017-11-02_04:40:49.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005812
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andreas Harst
SpeciesList	scientific name: Corynebacterium glutamicum ATCC 13032; NCBI TaxID: 196627;
ModificationList	Oxidation
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-31 00:13:04	ID requested
⏵ 1	2017-11-02 04:40:50	announced

Harst A, Albaum SP, Bojarzyn T, Tr, ö, tschel C, Poetsch A, Proteomics of FACS-sorted heterogeneous Corynebacterium glutamicum populations. J Proteomics, 160():1-7(2017) [pubmed]

curator keyword: Biological

submitter keyword: Corynebacterium glutamicum, BCAA producer, cellular heterogeneity, FACS, proteome analysis, label-free quantification

PD. Dr. Ansgar Poetsch
contact affiliation	School of Biomedical and Healthcare Sciences B427 Portland Square Plymouth University Drake Circus, Plymouth Devon PL4 8AA United Kingdom
contact email	ansgar.poetsch@ruhr-uni-bochum.de
lab head
Andreas Harst
contact affiliation	Ruhr University Bochum
contact email	andreas.harst@ruhr-uni-bochum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD005812

PRIDE project URI

• PRIDE
  1. PXD005812
     1. Label: PRIDE project
     2. Name: Proteomics of FACS-sorted heterogeneous Corynebacterium glutamicum populations