PXD005796

PXD005796 is an original dataset announced via ProteomeXchange.

Title	Membrane-bound TGF-beta on tumor-associated mesenchymal stem-like cells initiates epithelial-to-mesenchymal transition in human colorectal cancer
Description	Growing evidence indicates that tumor-associated stroma plays a negative role in human colorectal cancer (CRC). Nature of specific stromal cell populations involved and mechanisms underlying their negative impact remain to be fully understood. In this study we describe the expansion from human primary CRCs of a mesenchymal cell population, referred to as tumor-associated stromal cells (TASCs), resembling bone marrow-derived mesenchymal stem cells (BM-MSCs) in morphology, phenotypes and differentiation potential. We found that, upon co-culture with tumor cells, TASCs acquire membrane-bound TGF-mbTGF-expression, a phenomenon mediated by v6 integrin. MbTGF-expression proved to be critical for triggering epithelial-to-mesenchymal transition (EMT) in tumor cells, eventually leading to enhanced dissemination of circulating tumor cells and increased metastasis formation, in an orthotopic mouse model. Our data identify CRC-associated mesenchymal stem-like cells as critical EMT initiators and suggest mbTGF- as potential novel therapeutic target.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:36:18.292.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005796
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex; Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-27 05:27:29	ID requested
1	2022-05-19 01:42:02	announced
⏵ 2	2024-10-22 05:36:24	announced	2024-10-22: Updated project metadata.

10.6019/PXD005796;

10.3390/cancers14082024;

Mele V, Basso C, Governa V, Glaus Garzon JF, Muraro MG, D, ä, ster S, Nebiker CA, Mechera R, Bolli M, Schmidt A, Geiger R, Spagnoli GC, Christoforidis D, Majno PE, Borsig L, Iezzi G, Identification of TPM2 and CNN1 as Novel Prognostic Markers in Functionally Characterized Human Colon Cancer-Associated Stromal Cells. Cancers (Basel), 14(8):(2022) [pubmed]

curator keyword: Biomedical

submitter keyword: stem cells,TMT, LC-MS/MS, colorectal cancer

Alexander Schmidt
contact affiliation	Biozentrum, University of Basel, Klinglebergstrasse 50/70, CH-4056 Basel
contact email	alex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliation	Proteomics Core Facility
contact email	alex.schmidt@unibas.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD005796

PRIDE project URI

• PRIDE
  1. PXD005796
     1. Label: PRIDE project
     2. Name: Membrane-bound TGF-beta on tumor-associated mesenchymal stem-like cells initiates epithelial-to-mesenchymal transition in human colorectal cancer