PXD005787

PXD005787 is an original dataset announced via ProteomeXchange.

Title	Time-resolved metabolomics and proteomics of Brassica napus guard cells under low CO2 concentration
Description	In order to discover new nodes and edges for low CO2-induced stomata closure, we applied hyphenated mass spectrometry (MS)-based metabolomics and proteomics approaches to analyze short-term low CO2 responses in B. napus (canola) guard cells. A total of 411 metabolites were quantified. We observed decreased trends of primary metabolites biosynthesis (e.g., most common amino acids, nucleotides, and sugars), and changes in the levels of osmoregulators such as sucrose, malate, and mannitol showed increased trends at both early and late time points. Unlike under elevated CO2 levels, JA biosynthesis was not altered. Instead, phytohormones that induces stomatal opening, including cytokinins, auxins, GA4, as well as melatonin increased at early time points. This study also highlights the utility of single cell-type metabolomics in discovering and testing new nodes and edges in cellular signaling and metabolic networks.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_10:20:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ning Zhu
SpeciesList	scientific name: Brassica napus (Rape); NCBI TaxID: 3708;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-26 02:54:01	ID requested
⏵ 1	2018-10-26 10:20:16	announced

Geng S, Yu B, Zhu N, Dufresne C, Chen S, . Front Mol Biosci, 4():51(2017) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP)

submitter keyword: Guard cell, Metabolomics, Proteomics, Carbon dioxide, Phytohormones, Stomatal movement

Ning Zhu
contact affiliation	University of Florida
contact email	nzhu@ufl.edu
lab head
Ning Zhu
contact affiliation	UF
contact email	nzhu@ufl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD005787

PRIDE project URI

• PRIDE
  1. PXD005787
     1. Label: PRIDE project
     2. Name: Time-resolved metabolomics and proteomics of Brassica napus guard cells under low CO2 concentration